[3dem] Set value and actual magnification differ

[Sergej Masich]

Dear 3D-EM community,

We have observed a strange phenomenon in our old FEI CM200 microscope. For example, among many images acquired at the set value of 38 kX, there were a few images having actual magnification of 50 kX. The similar behaviour was observed for other magnifications as well. Our very experienced engineer could not explain the discrepancy. Can somebody advise what can have caused the problem?

Best regards,

Sergej
=============================
Dr. Sergej Masich
Dept. of Cell and Molecular Biology
Karolinska Institutet
171 77 Stockholm, Sweden
tel: (+)46 - 8 - 524 873 61
mobile: (+)46 - 736 - 833 693
e-mail: Sergej.Masich at ki.se<mailto:Sergej.Masich at ki.se>



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