[3dem] [EXTERNAL] Re: Vitrobot humidifier problem

Montserrat Samso montserrat.samso at vcuhealth.org
Mon Apr 23 11:19:22 PDT 2018

Dear All,

We had the same problem with our Vitrobot Mk 4. We disassembled the humidifier connector to the back of the machine and found some loose connections. After re-assembling properly this connection, the humidification worked fine again!

So it is worth a try.

Good luck,

Montserrat Samso, Ph.D.

Assistant Professor

Director, CryoEM Facility

Dept. Physiology and Biophysics

Virginia Commonwealth University

1101 E. Marshall St., RM 3-009

Richmond, VA 23298

Tel: (804) 828-8728

Fax: (804) 828-8492

E-mail: Montserrat.Samso at vcuhealth.org

From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Bowman, Valorie D <vdb at purdue.edu>
Sent: Monday, April 23, 2018 2:05:49 PM
To: Artemis Kosta; 3dem at ncmir.ucsd.edu
Subject: [EXTERNAL] Re: [3dem] Vitrobot humidifier problem

Dear All,

We recently have started having trouble getting our old Vitrobot humidifier to reach a high humidity when it's very dry in the lab.  It doesn't give any error messages like you've seen, it just doesn't go up.  I resolved it by using very hot water like we do for the CP3 sponge.  Not a very elegant solution, but it works!

David's sponge idea is a good one too if the little unit is completely kaput.



Valorie Bowman

vdb at purdue.edu
EM Facility Laboratory Manager/
Senior Research Electron Microscopist
Purdue Cryo EM Facility
Purdue University
From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Morgan, David Gene <dagmorga at indiana.edu>
Sent: Monday, April 23, 2018 11:09 AM
To: Artemis Kosta; 3dem at ncmir.ucsd.edu
Subject: Re: [3dem] Vitrobot humidifier problem


    We had a similar problem with a Mk 3.  After multiple attempts to fix various thing and some conversations with FEI, we eventually needed to have service come and fix the problem.  As I remember (this was several years ago), it required a new circuit board of some sort.  Sorry I can't be more helpful.

    politics is more difficult than physics.
                                             A. Einstein

            David Gene Morgan
        Electron Microscopy Center
             047D Simon Hall
             IU Bloomington
          812 856 1457 (office)
          812 856 3221 (3200)
From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Artemis Kosta <artemiskosta at gmail.com>
Sent: Monday, April 23, 2018 9:52 AM
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] Vitrobot humidifier problem

Dear colleagues,

we are experiencing a problem getting the Vitrobot MarkIV humidifier to work.
As soon as we try to increase the humidity in the chamber the system gives a message saying "empty" (in the humidity panel).

We have tried emptying and refilling it again and removing any air bubble in the line but that has not fixed the problem.

When we detach the cylinder from the Vitrobot, the water doesn't splash..
Has anyone else had this problem and if so how did you solve it?

Do you have a technical manual? We never managed to get one from FEI.

Best regards
Artemis Kosta

Artemis Kosta
Service de microscopie
FR3479 Institut de Microbiologie de la Méditerranée
31 chemin Joseph Aiguier
13402 Marseille cedex 20
akosta at imm.cnrs.fr<mailto:akosta at imm.cnrs.fr>
0033 491 164055

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