[3dem] negative staining artifact

[Subhomoi Borkotoky]

Hi Linda,

I believe its your stain precipitate . I too had this problem once (my UA
was old). I used a mild centrifuge rotation and took the supernatant for
staining and it worked. I hope this will solve your issue.

Regards,

Subhomoi B.

On Mon, Sep 25, 2017 at 12:31 PM, Johanna Höög <johanna.hoog at gu.se> wrote:

> Hej Linda!
>
>
>
> I had similar precipitates a while ago and it took me forever to find the
> source. For us, it was that the oil filter on the glow discharger that
> needed to be changed. Try just putting an empty grid in the glow discharger
> and then have a look at it to exclude this possibility.
>
>
>
> Good luck!
>
>
>
> Johanna
>
>
>
> *From: *3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Linda Sandblad <
> linda.sandblad at umu.se>
> *Date: *Sunday 24 September 2017 23:27
> *To: *"3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
> *Subject: *[3dem] negative staining artifact
>
>
>
> Hi all experts!!
>
> I know, this do not look nice, but I would like to hear your opinion about
> this artifact. Tiny dark spots covering both sample and background, do you
> know what it is, were it comes from and how to avoid it? We have this bad
> background time to time from different labs…. It is a “normal” negative
> staining protocol: copper grids, formvar, carbon, glow discharge, UA.
>
> I’m happy for your suggestions, Best Linda
>
>
>
> Linda Sandblad
> ……………………………………………….
> Department of Molecular Biology
> Umeå University
> +46 709324936 <+46%2070%20932%2049%2036>
>
>
>
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>


-- 
Yours Sincerely,
--------------------------
SUBHOMOI BORKOTOKY,
Centre for Bioinformatics
Pondicherry University
Pondicherry,INDIA.

Alternate Email: subhomoi at bicpu.edu.in <subhomoi at mails.bicpu.edu.in>

https://scholar.google.co.in/citations?user=bJz7GokAAAAJ&hl=en
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