[3dem] 3dem Digest, Vol 121, Issue 32

Sanchaita Das sanchaita06 at gmail.com
Sun Sep 24 18:50:33 PDT 2017


I would reccomend using UF and washing before staining with water.


I think 99  times and I find nothing.
I stop thinking,
swim in silence,
and the truth comes to me.

- Albert Einstein -


On Sep 25, 2017 2:57 AM, <3dem-request at ncmir.ucsd.edu> wrote:

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>    1. negative staining artifact (Linda Sandblad)
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> ----------------------------------------------------------------------
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> Message: 1
> Date: Sun, 24 Sep 2017 21:25:01 +0000
> From: Linda Sandblad <linda.sandblad at umu.se>
> To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
> Subject: [3dem] negative staining artifact
> Message-ID: <DD2852F6-2D2D-41A8-9570-E93BEAFE065F at molbiol.umu.se>
> Content-Type: text/plain; charset="utf-8"
>
> Hi all experts!!
> I know, this do not look nice, but I would like to hear your opinion about
> this artifact. Tiny dark spots covering both sample and background, do you
> know what it is, were it comes from and how to avoid it? We have this bad
> background time to time from different labs?. It is a ?normal? negative
> staining protocol: copper grids, formvar, carbon, glow discharge, UA.
> I?m happy for your suggestions, Best Linda
> [cid:0CCDA9FC-AEBF-4621-B208-6A9BFB2DF2B3 at lan]
>
> Linda Sandblad
> ??????????????????.
> Department of Molecular Biology
> Ume? University
> +46 709324936
>
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