<div dir="auto">I would reccomend using UF and washing before staining with water.<br><br><div data-smartmail="gmail_signature"><br>I think 99 times and I find nothing.<br>I stop thinking,<br>swim in silence,<br>and the truth comes to me.<br><br>- Albert Einstein -<br> </div></div><div class="gmail_extra"><br><div class="gmail_quote">On Sep 25, 2017 2:57 AM, <<a href="mailto:3dem-request@ncmir.ucsd.edu">3dem-request@ncmir.ucsd.edu</a>> wrote:<br type="attribution"><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">Send 3dem mailing list submissions to<br>
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1. negative staining artifact (Linda Sandblad)<br>
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Message: 1<br>
Date: Sun, 24 Sep 2017 21:25:01 +0000<br>
From: Linda Sandblad <<a href="mailto:linda.sandblad@umu.se">linda.sandblad@umu.se</a>><br>
To: "<a href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a>" <<a href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a>><br>
Subject: [3dem] negative staining artifact<br>
Message-ID: <<a href="mailto:DD2852F6-2D2D-41A8-9570-E93BEAFE065F@molbiol.umu.se">DD2852F6-2D2D-41A8-9570-<wbr>E93BEAFE065F@molbiol.umu.se</a>><br>
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Hi all experts!!<br>
I know, this do not look nice, but I would like to hear your opinion about this artifact. Tiny dark spots covering both sample and background, do you know what it is, were it comes from and how to avoid it? We have this bad background time to time from different labs?. It is a ?normal? negative staining protocol: copper grids, formvar, carbon, glow discharge, UA.<br>
I?m happy for your suggestions, Best Linda<br>
[cid:0CCDA9FC-AEBF-4621-B208-<wbr>6A9BFB2DF2B3@lan]<br>
<br>
Linda Sandblad<br>
??????????????????.<br>
Department of Molecular Biology<br>
Ume? University<br>
<a href="tel:%2B46%20709324936" value="+46709324936">+46 709324936</a><br>
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End of 3dem Digest, Vol 121, Issue 32<br>
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