[3dem] parameters for K2 super-resolution

[Garry P Morgan]

thanks everyone!
all of your answers have been super helpful.  we’ll stay away from the lower magnifications and focus on getting better quality images at 31000x.
cheers,
Garry


On Sep 20, 2017, at 11:47 AM, Zongli Li <zongli.li at gmail.com<mailto:zongli.li at gmail.com>> wrote:

Hi Garry,

When collecting SPA images with K2 summit, we always use super-resolution mode at a nominal mag of 31,000x for most samples. The image quality from 2x binned super-resolution images is better than normal counting mode image without binning. The dose rate is 8 e-/physical pixel/s (2 e-/super-resolution pixel/s, same as Marcus mentioned). Normally 40-50 frames per movie stack with sub-frame time of 200/250 ms.

Hope this helps,

Zongli

On Wed, Sep 20, 2017 at 11:22 AM, Garry P Morgan <Garry.Morgan at colorado.edu<mailto:Garry.Morgan at colorado.edu>> wrote:
Hi,

we’ve been collection SPA images with a K2 Summit on a Tecnai F30; so far we have only used counting mode at a nominal magnification of 31,000x.  we’d like to try using super-resolution at a lower mag (i.e., nominal mag of 20,000x) to get a wider field of view with a roughly equivalent pixel size.  i’m wondering if anyone is doing this as a standard for their SPA data acquisition? and if so, how are you setting up your acquisition parameters? i.e., frame time and number of frames.  because the dose is so much lower at the lower mag, it takes a significantly longer total acquisition time (20-30 seconds) to achieve our desired sample dose. in order to keep the number of frames in the movie stack to ~40 (so that the movie stack file size isn’t too large), we would need to increase the frame exposure time to between 0.5 and 1.0 sec. however, i’m worried this longer frame time would incorporate too much drift and essentially nullify the whole point of using the K2 and drift correction. i would appreciate any thoughts and advice on this.

one more question…Gatan recommends a dose rate no more than 10e-/ubpxel/sec going to the K2 while in counting mode. i  believe we were told the dose rate should be 2.5e-/ubpixel/sec for super-resolution mode.  can anyone tell me if this is true, or is the dose rate of 10e-/ubpixel/sec in regular counting mode actually the same as 2.5e- dose rate in super-res mode because of the 0.5x binning?  in other words, can i use the same beam intensity for for unbinned counting mode and super-res mode?

thanks,
Garry

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