[3dem] gold fiducials

[Wright, Elizabeth R.]

Hi Daniel,

We use these…
BSA-treated tracers, EM grade (EMS, cat. no. 25486).
You can also make your own BSA-treated gold, protocols outlined in Iancu et al., Nature Protocols (2006).

Good luck!

Liz

----
Elizabeth R. Wright, PhD
Associate Professor
Emory University
(404) 727-4665
erwrigh at emory.edu


From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Levy Daniel <Daniel.Levy at curie.fr>
Date: Thursday, September 14, 2017 at 8:03 AM
To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
Subject: [3dem] gold fiducials

Dear All,
The 10 nm ProteinA gold fiducials that we use in cryo-ET experiments strongly interact with one of our proteins of interest. Do you have any suggestions for a 10 or 6nm gold fiducials ?
Best
Daniel Lévy

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