[3dem] gold fiducials

Wright, Elizabeth R. erwrigh at emory.edu
Thu Sep 14 05:35:49 PDT 2017

Hi Daniel,

We use these…
BSA-treated tracers, EM grade (EMS, cat. no. 25486).
You can also make your own BSA-treated gold, protocols outlined in Iancu et al., Nature Protocols (2006).

Good luck!


Elizabeth R. Wright, PhD
Associate Professor
Emory University
(404) 727-4665
erwrigh at emory.edu

From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Levy Daniel <Daniel.Levy at curie.fr>
Date: Thursday, September 14, 2017 at 8:03 AM
To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
Subject: [3dem] gold fiducials

Dear All,
The 10 nm ProteinA gold fiducials that we use in cryo-ET experiments strongly interact with one of our proteins of interest. Do you have any suggestions for a 10 or 6nm gold fiducials ?
Daniel Lévy


This e-mail message (including any attachments) is for the sole use of
the intended recipient(s) and may contain confidential and privileged
information. If the reader of this message is not the intended
recipient, you are hereby notified that any dissemination, distribution
or copying of this message (including any attachments) is strictly

If you have received this message in error, please contact
the sender by reply e-mail message and destroy all copies of the
original message (including attachments).
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20170914/18d38927/attachment-0001.html>

More information about the 3dem mailing list