[3dem] 3dem Digest, Vol 122, Issue 69

[Qiaohui Yang]

Dear all,
I have attached an EM image here for an example. Note the bubble like structures surrounding the cells. Thank you so much for your help!
Many thanks,Qiaohui 

    On Tuesday, 17 October 2017, 3:00, "3dem-request at ncmir.ucsd.edu" <3dem-request at ncmir.ucsd.edu> wrote:
 

 Send 3dem mailing list submissions to
    3dem at ncmir.ucsd.edu

To subscribe or unsubscribe via the World Wide Web, visit
    https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
or, via email, send a message with subject or body 'help' to
    3dem-request at ncmir.ucsd.edu

You can reach the person managing the list at
    3dem-owner at ncmir.ucsd.edu

When replying, please edit your Subject line so it is more specific
than "Re: Contents of 3dem digest..."


Today's Topics:

  1. Gatan Multi-Specimen Single Tilt Cryo-Transfer Holder
      (Roberto Marotta)
  2. Re: Needing expert advice on cryosectioning for Tokuyasu
      method) (Vlad Speransky)


----------------------------------------------------------------------

Message: 1
Date: Mon, 16 Oct 2017 14:05:34 +0000
From: Roberto Marotta <Roberto.Marotta at iit.it>
To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
Subject: [3dem] Gatan Multi-Specimen Single Tilt Cryo-Transfer Holder
Message-ID:
    <394E95EBDD7E574B8D2123882082A58E6D13E229 at IITMXWGE015.iit.local>
Content-Type: text/plain; charset="us-ascii"

Hi,
I'm evaluating the Gatan 910 multi-specimens cryo-transfer holder we will eventually use in a Tecnai G2 F20 TWEEN microscope equipped with an automatic cryobox.
Any information you can provide me on this holder would be greatly appreciated.
Best,
Roberto Marotta

**************************************************************************
Roberto Marotta, PhD
Electron Microscopy Facility
Istituto Italiano di Tecnologia, IIT
Via Morego 30, 16163 Genoa, Italy
email: roberto.marotta at iit.it
t: +39 010 71781 897
f: +39 010 71781 236
P Please consider the environment before printing this e-mail.

-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20171016/5065b293/attachment-0001.html>

------------------------------

Message: 2
Date: Mon, 16 Oct 2017 07:57:58 -0700
From: "Vlad Speransky" <vlad_speransky at tedpella.com>
To: <3dem at ncmir.ucsd.edu>
Subject: Re: [3dem] Needing expert advice on cryosectioning for
    Tokuyasu    method)
Message-ID: <02f201d3468f$284f0010$78ed0030$@tedpella.com>
Content-Type: text/plain; charset="utf-8"

Hi Qiaohui,

 

Is there an image you could share? Your description suggests the methylcellulose possibly getting old, but could be other things. 

 

Best wishes,

Vlad

 

Vlad Speransky, PhD

Life Sciences Product Specialist

Ted Pella, Inc.

 <http://www.tedpella.com/> http://www.tedpella.com/ 

530-243-2200 ext. 266

Cell 530-768-3953

 <mailto:vlad_speransky at tedpella.com> vlad_speransky at tedpella.com

 

 

 

 

From: 3dem [mailto:3dem-bounces at ncmir.ucsd.edu] On Behalf Of qiaohui.yang at ymail.com
Sent: Sunday, October 15, 2017 2:28 AM
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] Needing expert advice on cryosectioning for Tokuyasu method)

 

Hi everyone,

 

 

I have been working on trying to master the technique of cryosectioning and immunogoldlabelling. My embedded sections in (mixture of 1% methycellulose and 1.15UA) on final image look good but there seems to many round blobs like bubbles that persist on/under my sections. Would appreciate if experts in this technique would be able to give some advice on how to rectify this. Thanks!

 

Kind regards to all,

Qiaohui

Sent from Yahoo Mail on Android <https://overview.mail.yahoo.com/mobile/?.src=Android> 

-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20171016/11820e8e/attachment-0001.html>

------------------------------

Subject: Digest Footer

_______________________________________________
3dem mailing list
3dem at ncmir.ucsd.edu
https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem


------------------------------

End of 3dem Digest, Vol 122, Issue 69
*************************************


   
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20171017/f5620323/attachment-0001.html>
-------------- next part --------------
A non-text attachment was scrubbed...
Name: Nickel grid 1_02.tif
Type: image/tiff
Size: 17505816 bytes
Desc: not available
URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20171017/f5620323/attachment-0001.tif>