[3dem] 2 positions in UK: London and Sheffield

Elena Orlova e.orlova at mail.cryst.bbk.ac.uk
Thu Oct 5 09:58:04 PDT 2017

*Dear colleagues*

We are seeking two motivated and creative Post Doctoral Researchers for 
structural and molecular biology studies to understand how DNA 
replication is initiated and regulated in the cell cycle. The research 
will be performed by the team led by Prof Elena Orlova (ISMB, Birkbeck, 
London) and Dr. Cyril Sanders (University of Sheffield). In the lab by 
Dr Sanders the discrete protein-DNA complexes will be assembled, 
purified and characterised for their distortions, melting and then 
unwinding of the DNA double helix. Structures of replication complexes 
will be determined using cryo electron microscopy (cryo-EM) in the lab 
of Prof Orlova at Birkbeck College (ISMB) in London. The opportunities 
offered by the “resolution revolution” in cryo-EM make this is an 
exciting time to be involved in mechanistic biochemistry. The full-time 
posts are funded by the Biotechnology and Biological Sciences Research 
Council (BBSRC) and are available for a period of 3 years.

*_Post 1_*

*Post Doctoral Researcher - Structural Biology(Ref: **12339**)*

*Postdoctoral positions in cryo-EM structural biology at **Institute of 
Structural and Molecular Biology (ISMB), Birkbeck College, London*****

*Department of Biological Sciences*

*Full time, fixed term appointment for up to 3 years *

A postdoctoral position is available for cryo-EM analysis of complexes 
formed by two viral proteins, E1 and E2 and a specific dsDNA sequence to 
understand replication initiation. Cryo-EM and single-particle analysis 
(SPA) allow visualisation of particles in solution under near-native 
conditions. The specific objectives are to determine the structure of 
the E1E2-ori pre-initiation complex and reveal the ATP-dependent 
conformational changes in the protein and DNA, to derive a 
high-resolution cryo-EM structure of the E1 helicase bound to a 
replication fork junction (RFJ)-like DNA substrate. It is important for 
both experimental design and interpretation of results to have active 
scientific interactions with the collaborating laboratories (Dr. C. 
Sanders, University of Sheffield).The applicant will interact with 
members of the Birkbeck Electron Microscopy group 

Applicants should have a Ph.D. (or equivalent) and be accomplished in 
biochemistry and structural biologyor a related area, and have relevant 
research publications. Expertise in cryo-EM structure determination 
would be especially desirable.

The laboratory is equipped with a high end Polara microscope and three 
other Tecnai microscopes including a 200 kV FEG system. Our Polara has a 
K2 direct electron detector and F20 has a DE camera s that provide high 
resolution data. Funding has been obtained to purchase in Titan Krios 
with direct electron detector. The EM lab has the associated facilities 
for sample preparation, including a vitrobot, and a dedicated linux 
cluster and graphics workstations for image processing. The Birkbeck EM 
lab is a major focus for EM research and training: we host an EMBO 
course on cryo EM single particle analysis every 2 years, and also an 
increasing number of successful research fellows.

For details and application forms please see: https://tinyurl.com/yda9xurm

Do not forget to read Further Information.pdf 

* The closing date for completed applications is midnight on 5^st 
November^, 2017*

*Interviews will be held on Monday 20 November 2017*

For further information on this opportunity contact Prof Elena Orlova 
(e.orlova at mail.cryst.bbk.ac.uk <mailto:e.orlova at mail.cryst.bbk.ac.uk>).

*_Post 2_*

*Post Doctoral Researcher - Molecular Biology(Ref: **UOS017363**)*

*Postdoctoral positions in molecular biology at **University of 

*Department of **Oncology & Metabolism***

*Full time, fixed term appointment for up to 3 years *

The vacancy can be viewed and applied for via the University's job 
webpages - https://www.sheffield.ac.uk/jobs ("Current vacancies" on 
left-hand side, search for job reference UOS017363).**

Please look at site: jobs.ac.uk <http://jobs.ac.uk> - 

*The closing date for completed applications is midnight on 20^st 
October, 2017*

For further information on this opportunity contact Dr Cyril Sanders 
c.m.sanders at sheffield.ac.uk )


Prof. Elena Orlova
Crystallography, Institute for Structural and Molecular Biology,
Department of Biological Sciences
Birkbeck,  University of London,
Malet Street,
Tel: (+44) (0)20 7631 6845
Fax: (+44) (0)20 7631 6803
e-mail: e.orlova at mail.cryst.bbk.ac.uk

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