[3dem] Microtubule in vitro polymerisation

[Prashant Singh]

Hi Linda,

It might be worth trying tubulin from PurSolutions - it is cycled and cryopreserved. 

You can find more info here: https://puresoluble.com/cycled-tubulin/ <https://puresoluble.com/cycled-tubulin/>

Thank you
Prash

*In the interest of full disclosure, I have a vested interest in PurSolutions.


> On Nov 9, 2017, at 3:33 PM, Linda Sandblad <linda.sandblad at umu.se> wrote:
> 
> Hi!! I must mention, at the EM facility we have many beautiful samples, it’s just that I often think the artifacts are more interesting to discuss ;-)
> 
> This is a question especially to all of you microtubule experts; When our students prepare mt from Cytoskeleton bovine tubulin it sometimes looks perfect, but sometimes it is all covered by those huge blobs, what do you think cause this artefact??
> 
> 
> 
> Happy to hear your thoughts! Best Linda
> 
> <170831_N5_MT01_0009_reduced.jpg>
> 
> 
> Linda Sandblad
> 
> Department of Molecular Biology
> Umeå University
> Tel: +46 709324936
> 
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