[3dem] Dose-symmetric tilt scheme for electron cryo tomography

[Davide Floris]

Dear all,
I’m wondering if any of you involved in electron cryo tomography has implemented the dose-symmetric tilt scheme from 
“Implementation of a cryo-electron tomography tilt-scheme optimized for high resolution subtomogram averaging” (Hagen WJ, Wan W, Briggs JA; JSB, 197(2):191-198; Feb 2017) 
to use longer exposure times as the tilt angle increases. In latitude it’s possible to do this by selecting the option “1/cosine^n” in the exposure time parameters, but of course it is not possible to use a symmetric acquisition.
Has anybody tried to modify the SerialEM macro in this sense?
Thanks a lot for the kind attention.
Best regards,

Davide Floris
_________________________________________________

Davide Floris
PhD student
Max Planck Institute of Biophysics
Structural Biology Department
Max-von-Laue Straße 3
60438 Frankfurt am Main
Germany

(L2.090): +49-69-6303-3037
(B2.205): +49-69-6303-3049

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