[3dem] Liposomes in ice

[Terje Dokland]

Maybe you didn't get that many liposomes in the first place? We have had similar problems with extruded liposomes. It seemed that a lot of the lipid did not form liposomes at all, but remained in micellar form, contaminating the background. 
 
On May 12, 2017, at 9:30 AM, Reza Khayat wrote:

> Hi,
> 
> We are preparing extruded liposomes (200nm). Problem is we're getting very few liposomes in ice. The starting lipid concentration (DOPG:https://avantilipids.com/product/840475/) is 4mg/ml.
> 
> Reza
> 
> Reza Khayat, PhD
> Assistant Professor 
> City College of New York
> Department of Chemistry
> New York, NY 10031
> From: Ludtke, Steven J <sludtke at bcm.edu>
> Sent: Friday, May 12, 2017 10:27 AM
> To: Reza Khayat
> Subject: Re: [3dem] Liposomes in ice
>  
> You'll have to define exactly what you mean by liposomes. Extruded SUVs are one of our favorite test/training specimens because they are so easy to prepare and freeze. When most people say liposome, they mean something much larger. You'll need to give some details if you want useful advice. What are the lipid constituents, how large are the liposomes, how are they prepared, etc.   Would also be useful to see a representative image or two.
> 
>> On May 12, 2017, at 9:18 AM, Reza Khayat <rkhayat at ccny.cuny.edu> wrote:
>> 
>> Hi,
>> 
>> We're trying to visualize liposomes in ice but are having some serious difficulty. Can anyone suggest some tricks that may be helpful? Thanks.
>> 
>> Best wishes,
>> Reza
>> 
>> Reza Khayat, PhD
>> Assistant Professor 
>> City College of New York
>> Department of Chemistry
>> New York, NY 10031
>> 
>> 
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> 
> ----------------------------------------------------------------------------
> Steven Ludtke, Ph.D.
> Professor, Dept of Biochemistry and Mol. Biol.         (www.bcm.edu/biochem)
> Co-Director National Center For Macromolecular Imaging        (ncmi.bcm.edu)
> Co-Director CIBR Center                          (www.bcm.edu/research/cibr)
> Baylor College of Medicine                             
> sludtke at bcm.edu
> 
> 
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