<html><head><base href="x-msg://229/"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">Maybe you didn't get that many liposomes in the first place? We have had similar problems with extruded liposomes. It seemed that a lot of the lipid did not form liposomes at all, but remained in micellar form, contaminating the background. <div> <br><div><div>On May 12, 2017, at 9:30 AM, Reza Khayat wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite"><span class="Apple-style-span" style="border-collapse: separate; font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; font-size: medium; "><div dir="ltr" style="font-size: 12pt; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255); font-family: Calibri, Arial, Helvetica, sans-serif; "><div style="margin-top: 0px; margin-bottom: 0px; ">Hi,</div><div style="margin-top: 0px; margin-bottom: 0px; "><br></div><div style="margin-top: 0px; margin-bottom: 0px; ">We are preparing extruded liposomes (200nm). Problem is we're getting very few liposomes in ice. The starting lipid concentration (DOPG:<a href="https://avantilipids.com/product/840475/">https://avantilipids.com/product/840475/</a>) is 4mg/ml.</div><div style="margin-top: 0px; margin-bottom: 0px; "><br></div><div style="margin-top: 0px; margin-bottom: 0px; ">Reza<br></div><div style="margin-top: 0px; margin-bottom: 0px; "><br></div><div id="Signature"><div name="divtagdefaultwrapper" style="font-family: Calibri, Arial, Helvetica, sans-serif; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">Reza Khayat, PhD<div>Assistant Professor </div><div>City College of New York</div><div>Department of Chemistry</div><div>New York, NY 10031</div></div></div><div style="word-wrap: break-word; "><hr tabindex="-1" style="display: inline-block; width: 1028px; "><div id="divRplyFwdMsg" dir="ltr"><font color="#000000" face="Calibri, sans-serif" style="font-size: 11pt; "><b>From:</b><span class="Apple-converted-space"> </span>Ludtke, Steven J <<a href="mailto:sludtke@bcm.edu">sludtke@bcm.edu</a>><br><b>Sent:</b><span class="Apple-converted-space"> </span>Friday, May 12, 2017 10:27 AM<br><b>To:</b><span class="Apple-converted-space"> </span>Reza Khayat<br><b>Subject:</b><span class="Apple-converted-space"> </span>Re: [3dem] Liposomes in ice</font><div> </div></div><div>You'll have to define exactly what you mean by liposomes. Extruded SUVs are one of our favorite test/training specimens because they are so easy to prepare and freeze. When most people say liposome, they mean something much larger. You'll need to give some details if you want useful advice. What are the lipid constituents, how large are the liposomes, how are they prepared, etc. Would also be useful to see a representative image or two.<div class=""><br class=""><div><blockquote type="cite" class=""><div class="">On May 12, 2017, at 9:18 AM, Reza Khayat <<a href="mailto:rkhayat@ccny.cuny.edu" class="">rkhayat@ccny.cuny.edu</a>> wrote:</div><br class="Apple-interchange-newline"><div class=""><div class="" style="margin-top: 0px; margin-bottom: 0px; font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px; font-style: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; background-color: rgb(255, 255, 255); ">Hi,</div><div class="" style="margin-top: 0px; margin-bottom: 0px; font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px; font-style: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; background-color: rgb(255, 255, 255); "><br class=""></div><div class="" style="margin-top: 0px; margin-bottom: 0px; font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px; font-style: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; background-color: rgb(255, 255, 255); ">We're trying to visualize liposomes in ice but are having some serious difficulty. Can anyone suggest some tricks that may be helpful? Thanks.</div><div class="" style="margin-top: 0px; margin-bottom: 0px; font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px; font-style: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; background-color: rgb(255, 255, 255); "><br class=""></div><div class="" style="margin-top: 0px; margin-bottom: 0px; font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px; font-style: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; background-color: rgb(255, 255, 255); ">Best wishes,</div><div class="" style="margin-top: 0px; margin-bottom: 0px; font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px; font-style: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; background-color: rgb(255, 255, 255); ">Reza<br class=""></div><div class="" style="margin-top: 0px; margin-bottom: 0px; font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px; font-style: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; background-color: rgb(255, 255, 255); "><br class=""></div><div id="Signature" class="" style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px; font-style: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; background-color: rgb(255, 255, 255); "><div name="divtagdefaultwrapper" class="" style="font-family: Calibri, Arial, Helvetica, sans-serif; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">Reza Khayat, PhD<div class="">Assistant Professor </div><div class="">City College of New York</div><div class="">Department of Chemistry</div><div class="">New York, NY 10031</div></div></div><div class="" style="font-style: normal; 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margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font-style: normal; font-variant: normal; font-weight: normal; font-size: 12px; line-height: normal; font-family: Courier; ">Steven Ludtke, Ph.D.</div><div class="" style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font-style: normal; font-variant: normal; font-weight: normal; font-size: 12px; line-height: normal; font-family: Courier; ">Professor, Dept of Biochemistry and Mol. Biol. (<a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.bcm.edu_biochem&d=DgMFAg&c=4NmamNZG3KTnUCoC6InoLJ6KV1tbVKrkZXHRwtIMGmo&r=1DzJFW0v6TgEhkW1gy_-ke-RbtvS1fzEbD5_hcb9Up0&m=swGrDni_bnbj0SDRv5hphQJtdOkdoQ-0OVRvykOGmk0&s=7k0aceBmiZX_c1AiKvYmgdfvlof36yBIJ3cb4Sh6F1Q&e=" class="">www.bcm.edu/biochem</a>)</div><div class="" style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font-style: normal; font-variant: normal; font-weight: normal; font-size: 12px; line-height: normal; font-family: Courier; ">Co-Director National Center For Macromolecular Imaging <span class="Apple-converted-space"> </span>(<a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__ncmi.bcm.edu&d=DgMFAg&c=4NmamNZG3KTnUCoC6InoLJ6KV1tbVKrkZXHRwtIMGmo&r=1DzJFW0v6TgEhkW1gy_-ke-RbtvS1fzEbD5_hcb9Up0&m=swGrDni_bnbj0SDRv5hphQJtdOkdoQ-0OVRvykOGmk0&s=vMVLCZ2x3qws1sikvDenN7wwVKbc5CZdCoKuHKU36L8&e=" class="">ncmi.bcm.edu</a>)</div><div class="" style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font-style: normal; font-variant: normal; font-weight: normal; font-size: 12px; line-height: normal; font-family: Courier; ">Co-Director CIBR Center <span class="Apple-converted-space"> </span>(<a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.bcm.edu_research_cibr&d=DgMFAg&c=4NmamNZG3KTnUCoC6InoLJ6KV1tbVKrkZXHRwtIMGmo&r=1DzJFW0v6TgEhkW1gy_-ke-RbtvS1fzEbD5_hcb9Up0&m=swGrDni_bnbj0SDRv5hphQJtdOkdoQ-0OVRvykOGmk0&s=xOaWyEtNdhTND18DZlvEv0ukWg-ciP2Uu7s6uCcAA1Y&e=" class="">www.bcm.edu/research/cibr</a>)</div><div class="" style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font-style: normal; font-variant: normal; font-weight: normal; font-size: 12px; line-height: normal; font-family: Courier; ">Baylor College of Medicine<span class="Apple-converted-space"> </span> <span class="Apple-converted-space"> </span></div><div class="" style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font-style: normal; font-variant: normal; font-weight: normal; font-size: 12px; line-height: normal; font-family: Courier; "><a href="mailto:sludtke@bcm.edu" class="">sludtke@bcm.edu</a></div></div><div class=""><br class=""></div></div></span></div></span></div></span></div></span><br class="Apple-interchange-newline"></div><br class="Apple-interchange-newline"><br class="Apple-interchange-newline"></div><br class=""></div></div></div>_______________________________________________<br>3dem mailing list<br><a href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a><br><a href="https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem">https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem</a><br></div></span></blockquote></div><br></div></body></html>