[3dem] volta phase plate recovery time

Anchi Cheng acheng at nysbc.org
Fri Mar 24 11:20:14 PDT 2017

Hi, Wim,

Thanks for replying.  No, these are regular-spaced spots that show up after
using the patch position.  Similar to Figure 2 in Rado’s 2015 PNAS paper.
The spacing corresponds also the distance between the defined distance
between patches.  Incidentally, this phase plate is the cleanest I have ever
seen.  It was hard to find a feature on it to align.

We do have some burn spots disappeared after 1.5 month.  There were the
ones that was irradiated less.

I am hoping I get a number from you or others with phase plates longer than
we have how long you wait before using the same phase plate patch position



On Mar 24, 2017, at 4:15 AM, Wim Hagen <wim.hagen at embl.de<mailto:wim.hagen at embl.de>> wrote:

Hi Anchi,

Maybe it is contamination? I see some spots in some areas on our brand new phase plate, the contrast of the spots is reversed w.r.g. to a Volta patch. Here are some images with many bad spots and one 'quick and dirty fresh' Volta patch, 'above and below' plane:




On Thu, Mar 23, 2017 at 18:33, Anchi Cheng <acheng at nysbc.org> wrote:
Hi, all,

I am wondering what people’s experience in the length of time
they wait before they reuse a patch position on FEI volta phase plate.

We can still see rows of the different contrast (burn spot, we call it) at low mag
and very high defocus (-100 mm) after a month and a half.

If I return to a patch in 2 weeks, the phase shift development is faster.  In addition,
there is no guarantee that I am back at the exact position.


Simons Microscopy Microscopy Center
New York Structural Biology Center
3dem mailing list
3dem at ncmir.ucsd.edu<mailto:3dem at ncmir.ucsd.edu>

-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20170324/c139f808/attachment.html>

More information about the 3dem mailing list