[3dem] volta phase plate recovery time

Anchi Cheng acheng at nysbc.org
Thu Mar 23 10:33:02 PDT 2017

Hi, all,

I am wondering what people’s experience in the length of time
they wait before they reuse a patch position on FEI volta phase plate.

We can still see rows of the different contrast (burn spot, we call it) at low mag
and very high defocus (-100 mm) after a month and a half.

If I return to a patch in 2 weeks, the phase shift development is faster.  In addition,
there is no guarantee that I am back at the exact position.


Simons Microscopy Microscopy Center
New York Structural Biology Center

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