[3dem] FEI-VPP phase change to opposite direction

Sacha De Carlo sacha.decarlo at dectris.com
Wed Jan 25 23:32:52 PST 2017

Could it be that the holder, with the new VPP, is no longer sitting in the
back focal plane, where it should be?
In any case, these are things to check with you FEI service engineer.

All the best,

On Thu, Jan 26, 2017 at 6:11 AM, jamon <jamonshi at gmail.com> wrote:

> Dear Lister,
> We just changed our FEI Volta Phase Plate on Krios. But after activation,
> the phase changed to opposite direction, the FFT rings moves outward
> instead of inwards as it should be. The gold particles all looks all white
> as if in overfocus. I had never seen such behavior with previous two VPPs
> we had. Did anybody have similar experience? Thanks in advance.
> --
> Best Regards,
> Jian SHI
> Manager, Cryo Electron Microscopy Facility
> Center for BioImaging Science
> National University of Singapore
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> 3dem at ncmir.ucsd.edu
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Sacha De Carlo, Ph.D.
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Phone: +41 56 500 2190 | Mobile: +41 79 354 4740 | Email:
sacha.decarlo at dectris.com
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