[3dem] FEI-VPP phase change to opposite direction

Matthias Wolf matthias.wolf at oist.jp
Wed Jan 25 21:41:29 PST 2017

Hi Jian,

Is the heater on?
The power supply should show "CC".


> On Jan 26, 2017, at 14:12, jamon <jamonshi at gmail.com> wrote:
> Dear Lister,
> We just changed our FEI Volta Phase Plate on Krios. But after activation, the phase changed to opposite direction, the FFT rings moves outward instead of inwards as it should be. The gold particles all looks all white as if in overfocus. I had never seen such behavior with previous two VPPs we had. Did anybody have similar experience? Thanks in advance.
> -- 
> Best Regards,
> Jian SHI
> Manager, Cryo Electron Microscopy Facility
> Center for BioImaging Science
> National University of Singapore
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