[3dem] FFT question

[Thorsten Mielke]

Hi,

if the effect shows up with one out of two cryo holders only (is the 
room temperature holder giving good FFTs?) , than most likely something 
is wrong with that holder. One reason might be that the clip ring does 
not clamp the grid properly. Do you always use the same clip ring with 
each holder? Did you check the that holder at room temperature? If OK at 
RT, something might be wrong with the thermal bridge between the dewar 
and the tip. Is the cut-off always in the same direction?

Best,

Thorsten




Am 29.12.2017 um 20:23 schrieb Garry P Morgan:
> Hi,
> we’re getting consistently clipped FFT’s on our TF30…the attached 
> image is from a 50-frame, summed/aligned image of the carbon (of a 
> cryo-grid). the drift numbers are reasonably low, there is no 
> astigmatism.  just wondering if any of you can provide a possible 
> explanation of this?  it definitely looks like drift or some sort of 
> movement, right?  we have 2 Gatan 626 holders, and it seems to only be 
> happening with one of the holders…has anyone encountered a problem 
> like this associated with a holder?
> thanks,
> Garry
>
> unknown.jpg
>
>
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-- 
*******************************************
Dr. Thorsten Mielke
- Head Microscopy and Cryo-Electron Microscopy Group -

Max Planck Institute for Molecular Genetics
Ihnestr. 63-73
D-14195 Berlin
                            
Tel.: +49-(0)30 8413-1644
Fax: +49-(0)30 8413-1385
E-mail: mielke at molgen.mpg.de

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