[3dem] growing mammalian cells on TEM grids, 3D print solution
Janice Green Pennington
pennington2 at wisc.edu
Tue Nov 8 10:00:24 PST 2016
Please send me the .stl files! What a great solution!
Janice G Pennington
Research Specialist, HHMI
Institute for Molecular Virology
University of Wisconsin - Madison
pennington2 at wisc.edu
From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Giovanna Grandinetti <giovanna.grandinetti at gmail.com>
Sent: Tuesday, November 8, 2016 9:53 AM
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] growing mammalian cells on TEM grids, 3D print solution
Hello 3dem members,
I have had trouble with bending grids when I try to grow mammalian cells on them. To combat this problem, I made some grid holders with our 3D printer. A full description can be found here
Holders for growing mammalian cells on grids for electron microscopy | NCI Technology Transfer Center | TTC<https://techtransfer.cancer.gov/availabletechnologies/e-243-2016>
The technology is a tool used to hold transmission electron microscopy grids to grow adherent mammalian cells on and the 3D printing software to create the holder. The TEM cell grid holder solves the difficulty of lifting the TEM grid out of a plate without bending or damaging the grid. Researchers at the NCI seek licensing for the transmission electron microscopy grid holder.
and some images are attached. The good thing about them is that they work very well and make it easier to pick up grids without bending them, the downside is that since the grids are raised above the glass to allow tweezers to slide under them, it is more difficult to get good high-resolution fluorescent images. The smaller ones were designed for glass-bottomed Mattek dishes with 20mm glass diameter
35 mm Dish - MatTek Corporation<https://www.mattek.com/store/p35g-1-0-20-c-case/>
35 mm Dish, No. 1.0 Coverslip, 20 mm Glass Diameter, Uncoated MatTek glass bottom dishes combine the convenience of standard size 35 mm and 50 mm disposabl
and the larger one fits in either a Mattek 35mm dish or a 6 well plate.
If anyone is interested in trying these, I would be happy to send the .stl files so you can 3D print your own with your choice of material. I can also send a printed out version of either one in PLA. Alternatively, I can send the Blender files if anyone would like to make any adjustments to these.
-------------- next part --------------
An HTML attachment was scrubbed...
More information about the 3dem