[3dem] Vitrification in the presence of DMSO

[Bob]

Hi Mihaela,

I have worked on samples with actin and myosin with up to 0.1% concentrations of DMSO and had no issues with obtaining quality ice.


Bob Ashley
Electron Microscopist
Penn State College of Medicine
500 University Drive
Mail Code H093 Room C1726
Hershey, PA 17033
717-531-0003 ext. 289256




On May 19, 2016, at 11:49 AM, Mihaela Folea <mirela79ro at yahoo.com> wrote:

>   Dear colleagues,
> 
> I am planning to vitrify some samples containg DMSO (no detergent present, by the way). What is your experience? Where you able to get good, clean ice?
> 
> Thank you in advance.
> 
> 
> Mihaela Folea, PhD
> 
> Bionanoscience Department, Technical University of Delft
> The Netherlands 
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