<html><head><meta http-equiv="Content-Type" content="text/html charset=us-ascii"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">Hi Mihaela,<div><br></div><div>I have worked on samples with actin and myosin with up to 0.1% concentrations of DMSO and had no issues with obtaining quality ice.</div><div><br></div><div><br></div><div><div apple-content-edited="true"><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;"><div style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">Bob Ashley</div><div style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">Electron Microscopist<br>Penn State College of Medicine<br>500 University Drive<br>Mail Code H093 Room C1726<br>Hershey, PA 17033<br>717-531-0003 ext. 289256</div><div style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;"><br></div><div style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;"><br><br></div></div>
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<br><div><div>On May 19, 2016, at 11:49 AM, Mihaela Folea <<a href="mailto:mirela79ro@yahoo.com">mirela79ro@yahoo.com</a>> wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite"><div><div style="background-color: rgb(255, 255, 255); font-family: HelveticaNeue, 'Helvetica Neue', Helvetica, Arial, 'Lucida Grande', sans-serif; font-size: 12px;"><div id="yui_3_16_0_1_1463672674524_2903"> Dear colleagues,</div><div id="yui_3_16_0_1_1463672674524_2904"><br></div><div id="yui_3_16_0_1_1463672674524_2905">I am planning to vitrify some samples containg DMSO (no detergent present, by the way). What is your experience? Where you able to get good, clean ice?</div><div id="yui_3_16_0_1_1463672674524_2906"><br></div><div id="yui_3_16_0_1_1463672674524_2907">Thank you in advance.</div><div id="yui_3_16_0_1_1463672674524_2908"><br></div><div id="yui_3_16_0_1_1463672674524_2909"><br></div><div id="yui_3_16_0_1_1463672674524_2919">Mihaela Folea, PhD</div><div id="yui_3_16_0_1_1463672674524_2911"><br></div><div id="yui_3_16_0_1_1463672674524_2912">Bionanoscience Department, Technical University of Delft</div><div id="yui_3_16_0_1_1463672674524_2920">The Netherlands </div></div></div>_______________________________________________<br>3dem mailing list<br><a href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a><br>https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem<br></blockquote></div><br></div></body></html>