[3dem] 3dem Digest, Vol 107, Issue 14
Qiang Guo
guoq.tiger at gmail.com
Sun Jul 17 04:18:35 PDT 2016
Hi Sanchaita,
I used to use Ni-NTA nanogold for cryo samples, the type I used is 5 nm
Ni-NTA-Nanogold (Nanoprobes, NY, USA). They also have lager ones(10 or
15nm), which may be useful for negative stain.
Best
Qiang
2016-07-15 21:00 GMT+02:00 <3dem-request at ncmir.ucsd.edu>:
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> 1. Re: 3dem Ni-NTA nanogold and Negative stain EM (Sanchaita Das)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Fri, 15 Jul 2016 12:37:09 -0400
> From: Sanchaita Das <sanchaita06 at gmail.com>
> To: 3dem at ncmir.ucsd.edu
> Subject: Re: [3dem] 3dem Ni-NTA nanogold and Negative stain EM
> Message-ID:
> <CAPft5nJi-3mfxttH47=_db50oQtV=4=YR+QRhVn=SjM=n76=
> 9A at mail.gmail.com>
> Content-Type: text/plain; charset="utf-8"
>
> Dear All,
> Would love to know if anyone has used Ni-NTA nano beads to do EM,
> especially Negative stain EM. If yes may you share some tips about how to
> make it work to get some decent data colelction and where may I purchase it
> from.
> Thanks in advance,
> Sanchaita
>
> On Wed, Jul 13, 2016 at 3:00 PM, <3dem-request at ncmir.ucsd.edu> wrote:
>
> > Send 3dem mailing list submissions to
> > 3dem at ncmir.ucsd.edu
> >
> > To subscribe or unsubscribe via the World Wide Web, visit
> > https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
> > or, via email, send a message with subject or body 'help' to
> > 3dem-request at ncmir.ucsd.edu
> >
> > You can reach the person managing the list at
> > 3dem-owner at ncmir.ucsd.edu
> >
> > When replying, please edit your Subject line so it is more specific
> > than "Re: Contents of 3dem digest..."
> >
> >
> > Today's Topics:
> >
> > 1. Re: Correction: Tenure-track or tenured investigator position
> > at NHLBI, NIH - Deadline Nov 20, 2016 (Jenny Hinshaw)
> >
> >
> > ----------------------------------------------------------------------
> >
> > Message: 1
> > Date: Tue, 12 Jul 2016 16:36:14 -0400
> > From: Jenny Hinshaw <jennyh at helix.nih.gov>
> > To: 3dem at ncmir.ucsd.edu
> > Subject: Re: [3dem] Correction: Tenure-track or tenured investigator
> > position at NHLBI, NIH - Deadline Nov 20, 2016
> > Message-ID: <9533785E-F415-4C2F-9533-AFCE72A9C204 at helix.nih.gov>
> > Content-Type: text/plain; charset="utf-8"
> >
> > The deadline for the following position has been moved up to October 31,
> > 2016. I apologize for all the emails. Feel free to contact me with any
> > questions.
> >
> > Jenny
> >
> > > On Jul 12, 2016, at 4:14 PM, Jenny Hinshaw <jennyh at helix.nih.gov>
> wrote:
> > >
> > >
> > > Investigator
> > >
> > > Cryo-Electron Microscopy
> > >
> > > Division of Intramural Research
> > >
> > > National Heart, Lung and Blood Institute, National Institutes of Health
> > >
> > > Department of Health and Human Services
> > >
> > >
> > >
> > > The Division of Intramural Research (DIR), National Heart, Lung, and
> > Blood Institute (NHLBI), NIH is seeking an outstanding tenure-track or
> > tenured investigator to initiate and direct an independent research
> program
> > in basic science on the NIH campus in Bethesda. This position is
> > equivalent to Assistant Professor or Associate/Full Professor level (with
> > the possibility of tenure at appointment) depending on qualifications and
> > experience. We are specifically seeking applicants for the technical
> > development and applications of Cryo-Electron Microscopy.
> > >
> > > The DIR environment provides the opportunity to perform creative and
> > innovative science. The existing faculty is an outstanding group of
> > internationally recognized biomedical researchers covering a wide range
> of
> > basic and clinical research topics including biochemistry, biophysics,
> > structural biology, cell biology, developmental biology, genetics,
> > neuroscience, stem cell biology, and virology. More detailed information
> > about the Principal investigators at NHLBI DIR may be found at
> > http://www.nhlbi.nih.gov/research/intramural/researchers/pi <
> > http://www.nhlbi.nih.gov/research/intramural/researchers/pi>.
> > >
> > > In addition to a newly acquired cryo-EM infrastructure (an FEI Titan
> > Krios with an energy filter and Gatan K2 direct detector is on order),
> the
> > institute is committed to invest in new instrumentation, long-term
> > operating costs and support personnel to create a state-of-the-art
> cryo-EM
> > facility. A generous start-up package as well as a fully funded operating
> > and personnel budget for the full duration of the appointment will be
> > offered for the successful candidate?s individual research program.
> > Furthermore, the investigator will have access to many outstanding
> research
> > core facilities including optical and electron microscopy, biophysics,
> > proteomics, DNA sequencing and genomics, transgenic, bioinformatics, flow
> > cytometry, and pathology. More detailed information may be found at
> > http://www.nhlbi.nih.gov/research/intramural/researchers/core <
> > http://www.nhlbi.nih.gov/research/intramural/researchers/core>. These
> > resources provide a unique research atmosphere unconstrained by
> traditional
> > support systems at academic or private research institutions.
> > >
> > > Applicants must have a Ph.D. (or equivalent) with significant
> experience
> > in many aspects of cryo-electron microscopy and a record of independent
> > scientific productivity as evidenced by citable publications. Excellent
> > collaborative history is a plus. Salary and appointment will be
> > commensurate with qualifications and experience. More detailed
> information
> > about the NHLBI DIR may be found at
> > http://www.nhlbi.nih.gov/research/intramural/ <
> > http://www.nhlbi.nih.gov/research/intramural/>
> > > Applicants may be US citizens, resident aliens or non-resident aliens
> > with or eligible for a valid employment visa. Applications must be
> received
> > by October 31, 2016. Please submit a curriculum vitae, brief statement of
> > research interests and provide a list of three references in PDF or MS
> Word
> > format only (no paper applications will be accepted) to:
> > >
> > > Robert S. Balaban, Ph.D., Scientific Director, NHLBI
> > > c/o Dan Fogarty, IAMB General Administration Section Chief, NHLBI
> > > nhlbi_recruit at mail.nih.gov <mailto:nhlbi_recruit at mail.nih.gov>
> > >
> > >
> > > DHHS and NIH are Equal Opportunity Employers. Applications from women,
> > minorities and persons with disabilities are strongly encouraged.
> > >
> > >
> > > <NHLBI NIH Cryo-EM PI Search.pdf>
> > > _______________________________________________
> > > 3dem mailing list
> > > 3dem at ncmir.ucsd.edu
> > > https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
> >
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> > End of 3dem Digest, Vol 107, Issue 13
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>
> --
> "For one human being to love another! That is perhaps the most difficult of
> all tasks...The work for which all other work is but preparation"
> Rainer Maria Rilke
>
>
> *Sanchaita Das*
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--
Qiang (John) Guo
Department of Molecular Structural Biology
Max Planck Institute of Biochemistry
Am Klopferspitz 18
82152 Martinsried
Germany
Email: guoq.tiger at gmail.com
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