<div dir="ltr"><div><div><div>Hi Sanchaita,<br><br></div>I used to use Ni-NTA nanogold for cryo samples, the type I used is 5 nm
Ni-NTA-Nanogold (Nanoprobes, NY, USA). They also have lager ones(10 or 15nm), which may be useful for negative stain.<br><br></div>Best<br></div>Qiang <br></div><div class="gmail_extra"><br><div class="gmail_quote">2016-07-15 21:00 GMT+02:00 <span dir="ltr"><<a href="mailto:3dem-request@ncmir.ucsd.edu" target="_blank">3dem-request@ncmir.ucsd.edu</a>></span>:<br><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">Send 3dem mailing list submissions to<br>
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Today's Topics:<br>
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1. Re: 3dem Ni-NTA nanogold and Negative stain EM (Sanchaita Das)<br>
<br>
<br>
----------------------------------------------------------------------<br>
<br>
Message: 1<br>
Date: Fri, 15 Jul 2016 12:37:09 -0400<br>
From: Sanchaita Das <<a href="mailto:sanchaita06@gmail.com">sanchaita06@gmail.com</a>><br>
To: <a href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a><br>
Subject: Re: [3dem] 3dem Ni-NTA nanogold and Negative stain EM<br>
Message-ID:<br>
<CAPft5nJi-3mfxttH47=_db50oQtV=4=YR+QRhVn=SjM=n76=<a href="mailto:9A@mail.gmail.com">9A@mail.gmail.com</a>><br>
Content-Type: text/plain; charset="utf-8"<br>
<br>
Dear All,<br>
Would love to know if anyone has used Ni-NTA nano beads to do EM,<br>
especially Negative stain EM. If yes may you share some tips about how to<br>
make it work to get some decent data colelction and where may I purchase it<br>
from.<br>
Thanks in advance,<br>
Sanchaita<br>
<br>
On Wed, Jul 13, 2016 at 3:00 PM, <<a href="mailto:3dem-request@ncmir.ucsd.edu">3dem-request@ncmir.ucsd.edu</a>> wrote:<br>
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> Send 3dem mailing list submissions to<br>
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> 1. Re: Correction: Tenure-track or tenured investigator position<br>
> at NHLBI, NIH - Deadline Nov 20, 2016 (Jenny Hinshaw)<br>
><br>
><br>
> ----------------------------------------------------------------------<br>
><br>
> Message: 1<br>
> Date: Tue, 12 Jul 2016 16:36:14 -0400<br>
> From: Jenny Hinshaw <<a href="mailto:jennyh@helix.nih.gov">jennyh@helix.nih.gov</a>><br>
> To: <a href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a><br>
> Subject: Re: [3dem] Correction: Tenure-track or tenured investigator<br>
> position at NHLBI, NIH - Deadline Nov 20, 2016<br>
> Message-ID: <<a href="mailto:9533785E-F415-4C2F-9533-AFCE72A9C204@helix.nih.gov">9533785E-F415-4C2F-9533-AFCE72A9C204@helix.nih.gov</a>><br>
> Content-Type: text/plain; charset="utf-8"<br>
><br>
> The deadline for the following position has been moved up to October 31,<br>
> 2016. I apologize for all the emails. Feel free to contact me with any<br>
> questions.<br>
><br>
> Jenny<br>
><br>
> > On Jul 12, 2016, at 4:14 PM, Jenny Hinshaw <<a href="mailto:jennyh@helix.nih.gov">jennyh@helix.nih.gov</a>> wrote:<br>
> ><br>
> ><br>
> > Investigator<br>
> ><br>
> > Cryo-Electron Microscopy<br>
> ><br>
> > Division of Intramural Research<br>
> ><br>
> > National Heart, Lung and Blood Institute, National Institutes of Health<br>
> ><br>
> > Department of Health and Human Services<br>
> ><br>
> ><br>
> ><br>
> > The Division of Intramural Research (DIR), National Heart, Lung, and<br>
> Blood Institute (NHLBI), NIH is seeking an outstanding tenure-track or<br>
> tenured investigator to initiate and direct an independent research program<br>
> in basic science on the NIH campus in Bethesda. This position is<br>
> equivalent to Assistant Professor or Associate/Full Professor level (with<br>
> the possibility of tenure at appointment) depending on qualifications and<br>
> experience. We are specifically seeking applicants for the technical<br>
> development and applications of Cryo-Electron Microscopy.<br>
> ><br>
> > The DIR environment provides the opportunity to perform creative and<br>
> innovative science. The existing faculty is an outstanding group of<br>
> internationally recognized biomedical researchers covering a wide range of<br>
> basic and clinical research topics including biochemistry, biophysics,<br>
> structural biology, cell biology, developmental biology, genetics,<br>
> neuroscience, stem cell biology, and virology. More detailed information<br>
> about the Principal investigators at NHLBI DIR may be found at<br>
> <a href="http://www.nhlbi.nih.gov/research/intramural/researchers/pi" rel="noreferrer" target="_blank">http://www.nhlbi.nih.gov/research/intramural/researchers/pi</a> <<br>
> <a href="http://www.nhlbi.nih.gov/research/intramural/researchers/pi" rel="noreferrer" target="_blank">http://www.nhlbi.nih.gov/research/intramural/researchers/pi</a>>.<br>
> ><br>
> > In addition to a newly acquired cryo-EM infrastructure (an FEI Titan<br>
> Krios with an energy filter and Gatan K2 direct detector is on order), the<br>
> institute is committed to invest in new instrumentation, long-term<br>
> operating costs and support personnel to create a state-of-the-art cryo-EM<br>
> facility. A generous start-up package as well as a fully funded operating<br>
> and personnel budget for the full duration of the appointment will be<br>
> offered for the successful candidate?s individual research program.<br>
> Furthermore, the investigator will have access to many outstanding research<br>
> core facilities including optical and electron microscopy, biophysics,<br>
> proteomics, DNA sequencing and genomics, transgenic, bioinformatics, flow<br>
> cytometry, and pathology. More detailed information may be found at<br>
> <a href="http://www.nhlbi.nih.gov/research/intramural/researchers/core" rel="noreferrer" target="_blank">http://www.nhlbi.nih.gov/research/intramural/researchers/core</a> <<br>
> <a href="http://www.nhlbi.nih.gov/research/intramural/researchers/core" rel="noreferrer" target="_blank">http://www.nhlbi.nih.gov/research/intramural/researchers/core</a>>. These<br>
> resources provide a unique research atmosphere unconstrained by traditional<br>
> support systems at academic or private research institutions.<br>
> ><br>
> > Applicants must have a Ph.D. (or equivalent) with significant experience<br>
> in many aspects of cryo-electron microscopy and a record of independent<br>
> scientific productivity as evidenced by citable publications. Excellent<br>
> collaborative history is a plus. Salary and appointment will be<br>
> commensurate with qualifications and experience. More detailed information<br>
> about the NHLBI DIR may be found at<br>
> <a href="http://www.nhlbi.nih.gov/research/intramural/" rel="noreferrer" target="_blank">http://www.nhlbi.nih.gov/research/intramural/</a> <<br>
> <a href="http://www.nhlbi.nih.gov/research/intramural/" rel="noreferrer" target="_blank">http://www.nhlbi.nih.gov/research/intramural/</a>><br>
> > Applicants may be US citizens, resident aliens or non-resident aliens<br>
> with or eligible for a valid employment visa. Applications must be received<br>
> by October 31, 2016. Please submit a curriculum vitae, brief statement of<br>
> research interests and provide a list of three references in PDF or MS Word<br>
> format only (no paper applications will be accepted) to:<br>
> ><br>
> > Robert S. Balaban, Ph.D., Scientific Director, NHLBI<br>
> > c/o Dan Fogarty, IAMB General Administration Section Chief, NHLBI<br>
> > <a href="mailto:nhlbi_recruit@mail.nih.gov">nhlbi_recruit@mail.nih.gov</a> <mailto:<a href="mailto:nhlbi_recruit@mail.nih.gov">nhlbi_recruit@mail.nih.gov</a>><br>
> ><br>
> ><br>
> > DHHS and NIH are Equal Opportunity Employers. Applications from women,<br>
> minorities and persons with disabilities are strongly encouraged.<br>
> ><br>
> ><br>
> > <NHLBI NIH Cryo-EM PI Search.pdf><br>
> > _______________________________________________<br>
> > 3dem mailing list<br>
> > <a href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a><br>
> > <a href="https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem" rel="noreferrer" target="_blank">https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem</a><br>
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--<br>
"For one human being to love another! That is perhaps the most difficult of<br>
all tasks...The work for which all other work is but preparation"<br>
Rainer Maria Rilke<br>
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<br>
*Sanchaita Das*<br>
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</blockquote></div><br><br clear="all"><br>-- <br><div class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr">Qiang (John) Guo<br>Department of Molecular Structural Biology<br>Max Planck Institute of Biochemistry<br>Am Klopferspitz 18<br>82152 Martinsried<br>Germany<br>Email: <a href="mailto:guoq.tiger@gmail.com" target="_blank">guoq.tiger@gmail.com</a> <br></div></div>
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