[3dem] Vitrobot blotting on one side only?

[Ben Engel]

Hi Garry,

We routinely blot from one side with the Vitrobot when vitrifying cells.
On the side without the filter paper, we put a teflon sheet that is cut in
the same shape.   Some of us also put a second piece of teflon under the
filter paper to slow the blotting, but I am not sure how necessary that is.

Best,
Ben

On Fri, Jul 15, 2016 at 12:56 PM, Garry P Morgan <Garry.Morgan at colorado.edu>
wrote:

> Hello,
> I’d like to blot with only one filter paper on the Vitrobot.  Has anyone
> done this successfully?  If you have any tips or tricks could you please
> let me know?  I was thinking of using a piece of aclar film in place of the
> filter paper on the non-blotting side. I’m sure there are other approaches.
> Any advice would be appreciated.
> thanks,
> Garry
>
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