[3dem] Phase plate test dataset

[Radostin Danev]

Dear Colleagues,

 

As I mentioned at the 3DEM GRC I am happy to share a small subset of phase
plate images acquired with defocus.

I put the first 20 images of a 20S proteasome dataset on my Dropbox, here:
https://www.dropbox.com/sh/omjjhwhhqsjvsm6/AAAAg32yTzc4_CvrjtdQI_Raa?dl=0

There are also picked particle coordinates in *_automatch.star and CTF
parameters in *_ctffind3.log files.

Other data:

- accel. voltage 300 kV

- pixel size 1.054 A

- Cs 2.62 mm

- particle diameter 190 A

 

With D7 symmetry this subset of ~ 7,300 particles refines to ~ 2.9 A
resolution.

 

All the best,

 

Rado

 

 

===================================

Radostin Danev, Ph.D.

Max Planck Institute of Biochemistry
Department of Molecular Structural Biology
Am Klopferspitz 18
82152 Martinsried
Germany

e-mail: danev at biochem.mpg.de

phone: +49 (89) 8578-2651
fax: +49 (89) 8578-2641

===================================

 

 

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