[3dem] cryo-TEM on JEOL 1400

Anindito Sen emailanindito at yahoo.co.in
Tue Jan 12 20:31:58 PST 2016


Hi David,

Following up with Masa's comments, you can also use Gatan's

・MODEL 636 Double Tilt Liquid Nitrogen Cooling Holder
・MODEL 654 Single Tilt Straining Holder

and
    ・MODEL 626 Single Tilt Liquid Nitrogen CryoTransfer Holder.

Best Wishes
Andy 
 Anindito Sen (Ph.D)



 

      From: Masahide Kikkawa <mkikkawa at m.u-tokyo.ac.jp>
 To: 3dem at ncmir.ucsd.edu 
 Sent: Tuesday, 12 January 2016, 10:06
 Subject: Re: [3dem] cryo-TEM on JEOL 1400
   
Hi David,

 If the microscope is double-O-ring type ( I think it should ) and equipped with anti-contaminator device, any Gatan cryo-holder should work. 
We have two 622s, one 914 (tomography), and one CT-3500. According to Gatan, CT-3500 is slightly better in stability, but for screening it doesn’t matter.

> On Jan 12, 2016, at 09:36, David Belnap <dbelnap at cores.utah.edu> wrote:
> 
> We have a new JEOL JEM-1400 that has worked very well and is a popular microscope for imaging negative stain and thin-section specimens.  We are thinking of buying a cryo-holder so we can use the 1400 to screen cryo-TEM specimens.  Does anyone have any experience using the 1400 for cryo-TEM?  Your thoughts would be appreciated.
> 
> Best regards,
> 
> David
> 
> =======================================
> David M. Belnap
> Electron Microscopy Core Laboratory
> Department of Biochemistry
> Department of Biology
> 2501 SMBB
> University of Utah
> Salt Lake City, Utah 84112 USA
> Phone  801.585.1242
> FAX  801.587.3077
> David.Belnap at utah.edu
> =======================================
> 
> 
> 
> 
> 
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> 3dem at ncmir.ucsd.edu
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