[3dem] IHRSR++ and SPIDER 21.02

Johannes Haataja johannes.haataja at aalto.fi
Mon Feb 29 11:58:40 PST 2016


Dear all, 
	thank you for the replies. I now have an older version of spider.

Regarding IHRSR Prof. Egelman - what would the recommended way/tutorial
for learning to use IHRSR? 

My best,
 - J.

P.S. I guess ideally one would just read an article about the theory and
unix/linux man-pages of relevant command line tools and then inductively
reason how one must proceed to apply the method to the problem at hand.
Since I lack such a tenacity, I usually look for tutorials in order to
understand how the softwares/black boxes work. Also, I imagine that for
helical reconstruction, like for any inverse problem, there are many
different methods for recovering the quantit(y/ies) of interest and that
people usually are hesitant to openly aside with particular approach may
it be the right one or obviously the wrong one (e.g. Bayesian vs.
Frequentist interpretation of statistics) ;). 

 

ma, 2016-02-29 kello 12:13 -0500, Michael Radermacher kirjoitti:
> I would contact the people in Albany and also
> discuss with them the problem you are having
> with your version.
> 
> Michael
> 
> On 2/29/2016 11:46 AM, Johannes Haataja wrote:
> > Hi,
> > 	does anyone know where to obtain old versions of SPIDER, namely v.
> > 21.02? The oldest from download page is 21.11. The reason for asking is
> > that I need and older SPIDER version to test IHRSR++ v. 1.5 tutorial
> >
> > http://cryoem.ucsd.edu/wikis/software/start.php?id=ihrsr
> >
> > to exclude the possibility that the errors I run into (in the final
> > reconstruction step) have something to do with SPIDER version.
> >
> > My best,
> > 	- J.
> >
> >
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> 
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