[3dem] more on the odd CCD image

Jaap Brink jbrink at jeol.com
Thu Feb 4 16:40:51 PST 2016


Steve, if I recall that event correctly weren't there a lot of spikes in the FFT? I don't remember whether or not the real space image looked this obviously bad.

Jaap
----
Jacob Brink Ph.D.
TEM Product Manager Life Sciences
JEOL USA, Inc., Peabody MA 01960
(978) 317-9561

Sent from mobile device. Please excuse typos and this reply's brevity as I'm using all thumbs to type :-)

On Feb 4, 2016, at 7:27 PM, Ludtke, Steven J <sludtke at bcm.edu<mailto:sludtke at bcm.edu>> wrote:

Electrical noise due to a loose/bad cable?  I seem to recall a case where we had a bad cable connection due to being periodically "bumped" or somesuch. This is a bit more extreme than I recall seeing, but certainly if there is a grounding issue or a bad cable all sorts of electrical badness can occur. If you know the readout rate of the detector you can estimate the frequency of the noise you're seeing.

Of course it could be something else   :^)


----------------------------------------------------------------------------
Steven Ludtke, Ph.D.
Professor, Dept. of Biochemistry and Mol. Biol.                Those who do
Co-Director National Center For Macromolecular Imaging               ARE
Baylor College of Medicine                                     The converse
sludtke at bcm.edu<mailto:sludtke at bcm.edu>  -or-  stevel at alumni.caltech.edu<mailto:stevel at alumni.caltech.edu>               also applies
http://ncmi.bcm.edu/~stevel

On Feb 4, 2016, at 6:08 PM, David Gene Morgan <dagmorga at indiana.edu<mailto:dagmorga at indiana.edu>> wrote:

Hi,

   Someone pointed out that the image I attached to my original post was not passed by the mail server.  You can see that image at:

   http://iubemcenter.indiana.edu/oddImageFromUltrascan890.html

--
               David Gene Morgan
           Electron Microscopy Center
                047D Simon Hall
                IU Bloomington
             812 856 1457 (office)
             812 856 3221 (3200)
         http://iubemcenter.indiana.edu
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