[3dem] Opportunities at UVa

[Edward Egelman]

*Opportunities in Cryo-EM of Protein and Nucleoprotein Polymers at the 
University of Virginia.***The Egelman lab has developed the main method 
(IHRSR) that is now being used around the world for reconstructing 
helical polymers at high resolution. Current projects in the Egelman lab 
include the application and further development of these methods to 
helical viruses (1,2), bacterial secretion systems (3), designed 
polymers (4), and proteins involved in inflammation and innate immunity 
(5). A full publication list can be found at people.virginia.edu/~ehe2n. 
The facilities are exceptional, and include a Titan Krios equipped with 
a Falcon II direct electron detector. If interested please contact Ed 
Egelman at egelman at virginia.edu.

1          DiMaio, F. et al. A Virus that Infects a Hyperthermophile 
Encapsidates A-Form DNA. Science 348, 914-917 (2015).

2          DiMaio, F. et al. The molecular basis for flexibility in the 
flexible filamentous plant viruses. Nat Struct Mol Biol 22, 642-644 (2015).

3          Kudryashev, M. et al. Structure of the Type VI Secretion 
System Contractile Sheath. Cell 160, 952-962 (2015).

4          Egelman, E. H. et al. Structural plasticity of helical 
nanotubes based on coiled-coil assemblies. Structure 23, 280-289 (2015).

5          Lu, A. et al. Unified Polymerization Mechanism for the 
Assembly of ASC-Dependent Inflammasomes. Cell 156, 1193-1206 (2014).