[3dem] PhD and post-doc position for cryo-STEM development

[Michael Elbaum]

PhD and post-doctoral positions are available to work on scanning transmission electron microscope (STEM) imaging for cellular cryo-tomography. STEM offers a less explored, alternative mode to wide-field phase contrast TEM. Recent advances in our lab have demonstrated important advantages of STEM, including suitability to thicker specimens and the absence of contrast oscillations associated with defocus. (see [1]) Many modern cryo-TEM instruments are STEM-capable but to date their application to cryo-imaging has hardly been tested. We aim in particular to leverage the quantitative nature of dark-field STEM to image interpretation as well as to mapping of trace elements using a variety of cellular and reconstituted model systems. The approach draws significantly from materials science microscopy so candidates with a background in physics or materials science are welcome. Prior experience in advanced microscopy, structural, or cellular biology would be a significant advantage.

The Weizmann Institute Electron Microscopy Unit is a vibrant, versatile facility covering both life science and materials science applications. Altogether there are six transmission and three scanning microscopes, plus a dual beam FIB-SEM. Optical fluorescence microscopy including super-resolution (STORM) is integrated for correlative imaging, and a wide range of sample preparation tools is available.

For more information please contact Prof Michael Elbaum, michael.elbaum at weizmann.ac.il (http://www.weizmann.ac.il/materials/elbaum/) or Dr Sharon Wolf, sharon.wolf at weizmann.ac.il


[1] Wolf et al, Cryo-scanning transmission electron tomography of vitrified cells. Nature Methods 2014, doi:10.1038/nmeth.2842, chosen EMS Outstanding Paper of 2014.

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