[3dem] 3dem Digest, Vol 95, Issue 17
Li, Kunpeng
li2182 at purdue.edu
Fri Jul 24 12:22:31 PDT 2015
Hi Frank,
I tried on both CP3 and Vitrobot 1st generation, but same results.
Your sincerely
Kunpeng Li
Research Scholar
Biological Science Department
Office: (765) 496-9317 / Cell: (765) 421-5516
Hockmeyer Hall of Structural Biology, West Lafayette IN 47906
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From: 3dem [3dem-bounces at ncmir.ucsd.edu] on behalf of 3dem-request at ncmir.ucsd.edu [3dem-request at ncmir.ucsd.edu]
Sent: Friday, July 24, 2015 3:00 PM
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Subject: 3dem Digest, Vol 95, Issue 17
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Today's Topics:
1. Re: 3dem Digest, Vol 95, Issue 16 (Nijpels Frank (ID))
2. Staff scientist position at the Wadsworth Center
(Agrawal, Rajendra (HEALTH))
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Message: 1
Date: Thu, 23 Jul 2015 22:31:22 +0200
From: "Nijpels Frank (ID)" <frank.nijpels at maastrichtuniversity.nl>
To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
Subject: Re: [3dem] 3dem Digest, Vol 95, Issue 16
Message-ID:
<DF8BA3728269214BA42656B6026A5FCF051ADBB9F3 at UM-MAIL4109.unimaas.nl>
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Hi Li,
What kind of plunger do you use?
regards,
Frank
________________________________________
Van: 3dem [3dem-bounces at ncmir.ucsd.edu] namens 3dem-request at ncmir.ucsd.edu [3dem-request at ncmir.ucsd.edu]
Verzonden: donderdag 23 juli 2015 21:47
Aan: 3dem at ncmir.ucsd.edu
Onderwerp: 3dem Digest, Vol 95, Issue 16
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Today's Topics:
1. vitrified ice but not continuously, How to make it better?
(Li, Kunpeng)
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Message: 1
Date: Thu, 23 Jul 2015 10:27:09 +0000
From: "Li, Kunpeng" <li2182 at purdue.edu>
To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
Subject: [3dem] vitrified ice but not continuously, How to make it
better?
Message-ID:
<1160C903B1262A488C0DAECFE601E1AC1C11E1 at WPVEXCMBX03.purdue.lcl>
Content-Type: text/plain; charset="us-ascii"
Dear All,
Recently I want to optimize the freeze conditions of a protein complex, But No matter how I can change the conditions, I cannot get the thin and continuous vitrified ice. the mass of the protein complex is almost 900 kDa. and the carbon grid is Quantifoil 1/1, glow discharge time is 1.5 minutes, the humidity in the plunger chamber is more than 80%RH, and the ice thickness I estimated is near to 60 nm, I post the images from search mode to photo mode under CM200 with 1k*1K CCD. I tried a low salt buffer and longer blot time at this check round. The accurate number post on the attached images.
Do you confused with these similar problems once before? Could you give me some clues to improve it?
Thanks for any help.
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Message: 2
Date: Fri, 24 Jul 2015 15:38:59 +0000
From: "Agrawal, Rajendra (HEALTH)" <rajendra.agrawal at health.ny.gov>
To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
Subject: [3dem] Staff scientist position at the Wadsworth Center
Message-ID:
<BN1PR09MB076041BC048C85FC483B3D4CB810 at BN1PR09MB076.namprd09.prod.outlook.com>
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A permanent Staff Scientist position in cryo-EM is available at Wadsworth Center, NY State Department of Health, Albany, NY. We seek applications from highly motivated individuals specializing in various aspects of high-resolution electron microscopy. Responsibilities for this position include the maintenance of high-end electron microscopes and ancillary equipment, cryo-specimen preparation, data collection, and management. The new hire will be expected to actively support grant-funded projects and the development of new grant applications. Salary will be commensurate with experience.
We have excellent institutional support and infrastructure, including a state-of-the-art JEOL 3200 FSC/PP microscope with an in-column energy filter and a K2 Summit direct-electron detector camera. Interested applicants should submit a cover letter describing their relevant experience, curriculum vitae, and contact information for at least three references to wcphgc at health.ny.gov<mailto:wcphgc at health.ny.gov>, with "3D-EM" in the subject line. Applications will be accepted until the position is filled and reviewed as they are received. AA/EOE.
Thank you,
-Raj Agrawal
*****************************************
Rajendra K. Agrawal, Ph.D.
Research Scientist VI
Deputy Director
Division of Translational Medicine
Wadsworth Center
New York State Dept. of Health
Empire State Plaza
Albany, NY 12201-0509
Tel: 518 - 486-5797 / 474-8759
Fax: 518 - 474 5049
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