[3dem] Ribosome

[Franken, L.E.]

Hi Shebl,
We did a negative stain project on ribosomes a while back. The m&m contains
information on the buffers we used.

http://onlinelibrary.wiley.com/doi/10.1111/mmi.12468/pdf

best wishes,
Linda

On Wed, Jul 8, 2015 at 9:00 PM, <3dem-request at ncmir.ucsd.edu> wrote:

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>    1.  Ribosome - negative staining - help request
>       (Shebl, Bassem M. (MU-Student))
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> ----------------------------------------------------------------------
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> Message: 1
> Date: Wed, 8 Jul 2015 15:52:26 +0000
> From: "Shebl, Bassem M. (MU-Student)" <bmgmw7 at mail.missouri.edu>
> To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
> Subject: [3dem]  Ribosome - negative staining - help request
> Message-ID: <0D8CF88D-2275-417A-8354-15230E801E89 at mail.missouri.edu>
> Content-Type: text/plain; charset="utf-8"
>
> Dear all,
>
> I am a new student to the field of electron microscopy. As a part of a
> project to an electron microscopy course I am taking, we are trying to look
> at negatively stained ribosomes. Does anyone have experience regarding
> this? Buffers used, concentrations, stain used? I did search the literature
> few times to look for recent publications on the topic but didn?t come
> across anything useful. I started with 2% Uranyl Acetate in ?20 mM
> HEPES?KOH (pH 7.5), 6 mM MgCl2, 150 mM NH4Cl, 6 mM b-mercaptoethanol, 2 mM
> spermidine, and 0.1 mM spermine? with a concentration of 15 nM.
>
> Bassem Shebl
>
>
>
>
>
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