<div dir="ltr"><div>Hi Shebl,<br></div>We did a negative stain project on ribosomes a while back. The m&m contains information on the buffers we used.<br><div><div><br><a href="http://onlinelibrary.wiley.com/doi/10.1111/mmi.12468/pdf">http://onlinelibrary.wiley.com/doi/10.1111/mmi.12468/pdf</a> <br><br></div><div>best wishes,<br></div><div>Linda<br></div><div><div class="gmail_extra"><br><div class="gmail_quote">On Wed, Jul 8, 2015 at 9:00 PM, <span dir="ltr"><<a href="mailto:3dem-request@ncmir.ucsd.edu" target="_blank">3dem-request@ncmir.ucsd.edu</a>></span> wrote:<br><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex">Send 3dem mailing list submissions to<br>
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1. Ribosome - negative staining - help request<br>
(Shebl, Bassem M. (MU-Student))<br>
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Message: 1<br>
Date: Wed, 8 Jul 2015 15:52:26 +0000<br>
From: "Shebl, Bassem M. (MU-Student)" <<a href="mailto:bmgmw7@mail.missouri.edu">bmgmw7@mail.missouri.edu</a>><br>
To: "<a href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a>" <<a href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a>><br>
Subject: [3dem] Ribosome - negative staining - help request<br>
Message-ID: <<a href="mailto:0D8CF88D-2275-417A-8354-15230E801E89@mail.missouri.edu">0D8CF88D-2275-417A-8354-15230E801E89@mail.missouri.edu</a>><br>
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Dear all,<br>
<br>
I am a new student to the field of electron microscopy. As a part of a project to an electron microscopy course I am taking, we are trying to look at negatively stained ribosomes. Does anyone have experience regarding this? Buffers used, concentrations, stain used? I did search the literature few times to look for recent publications on the topic but didn?t come across anything useful. I started with 2% Uranyl Acetate in ?20 mM HEPES?KOH (pH 7.5), 6 mM MgCl2, 150 mM NH4Cl, 6 mM b-mercaptoethanol, 2 mM spermidine, and 0.1 mM spermine? with a concentration of 15 nM.<br>
<br>
Bassem Shebl<br>
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