[3dem] Ribosome - negative staining - help request

[Liu Zheng]

Hi Bassem,
 The  concentration suiting for negative-stain observation ranges from 50nM
to 100nM.
--Zheng

Zheng Liu Ph.D.
Postdoc, The Frank Lab
Department of Biochemistry & Molecular Biophysics
Columbia University
http://franklab.cpmc.columbia.edu/franklab/

On Wed, Jul 8, 2015 at 11:52 AM, Shebl, Bassem M. (MU-Student) <
bmgmw7 at mail.missouri.edu> wrote:

>  Dear all,
>
>  I am a new student to the field of electron microscopy. As a part of a
> project to an electron microscopy course I am taking, we are trying to look
> at negatively stained ribosomes. Does anyone have experience regarding
> this? Buffers used, concentrations, stain used? I did search the literature
> few times to look for recent publications on the topic but didn’t come
> across anything useful. I started with 2% Uranyl Acetate in “20 mM
> HEPES􏰄KOH (pH 7.5), 6 mM MgCl2, 150 mM NH4Cl, 6 mM b-mercaptoethanol, 2
> mM spermidine, and 0.1 mM spermine” with a concentration of 15 nM.
>
>  Bassem Shebl
>
>
>
>
>
>
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>
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