[3dem] THE SEVENTH BRAZIL SCHOOL FOR SINGLE PARTICLE CRYO-EM (SECOND ANNOUNCEMENT)

[Marin van Heel]

NOTE: We have experienced a very strong interest in the 7th Brazil 
school since its first announcement. The school is already likely to be 
oversubscribed so don't delay your registration!

_Second announcement:_


_THE SEVENTH BRAZIL SCHOOL FOR SINGLE PARTICLE CRYO-EM._

11-23 August 2016 (São Paulo, Brazil) (www.single-particles.org/school).

Our single-particle school is a unique intensive 12-day hands-on 
course/workshop, in which theory and practice are tightly intertwined. 
The understanding of the principles of cryo-EM data processing stands 
central in this event and a few pioneers will explain the approaches to 
the participants to the level where they can apply it themselves. The 
spectrum of topics covered suits attendees who are new to the field as 
well as those for whom the cryo-EM technique is of growing importance 
and who wish to acquire an in-depth understanding of its fundamental 
principles.

The final days of the meeting will be devoted to advanced topics such as 
high-resolution refinements and the processing of heterogeneous data 
sets ("4D cryo–EM”). Advanced topics will include:

1)Optimizing data collection with direct electrons detectors and 
exploiting movies.

2)Full data set automatic CTF correction.

3)Correction of anisotropic magnification.

4)Reaching atomic resolution without reference bias: 
alignment-by-classification (ABC) refinement procedures.

5)Unbiased "Random Startup" of 3D and 4D reconstructions.

6)4D refinement procedures for high resolution results using IMAGIC-4D 
and/or Frealign.

7)Fitting and refining atomic coordinates in high-resolution cryo-EM 
maps (~4Å or better) using programs like REFMAC and Coot.

8)Validation of the final results.

Kind regards,

Marin van Heel


-- 
================================================================

     Prof Dr Ir Marin van Heel

     Professor of Cryo-EM Data Processing

     Leiden University
     NeCEN Building Room 05.27
     Einsteinweg 55
     2333 CC Leiden
     The Netherlands
      
     Tel. NL: +31(0)715271424 // Mobile NL: +31(0)652736618
     Skype:    Marin.van.Heel
     email:  marin.vanheel(A_T)gmail.com
     and:    mvh.office(A_T)gmail.com

----------------------------------------------

     Emeritus Professor of Structural Biology

     Imperial College London
     Faculty of Natural Sciences
     Biochemistry Building (Room 512)
     South Kensington Campus
     London SW7 2AZ,  UK
     email:  m.vanheel(A_T)ic.ac.uk

     Tel. UK:   +44(0)2075945316 //Mobile: +44(0)7941540625

----------------------------------------------
     Visiting Professor at:

     Laboratório Nacional de Nanotecnologia - LNNano
     CNPEM/ABTLuS, Campinas, Brazil
     Brazilian mobile phone  +55-19-983189143

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