[3dem] [ccpem] on FSC curve (A can of worms...)

[Edward Egelman]

Since Marin brought up the can of worms metaphor, let me just add my own 
two bits: FSC has never been a measure of resolution (in the Rayleigh 
sense of an instrument's ability two resolve two points) but has been a 
measure of self-consistency. One can then argue about the statistics of 
this measure, but please keep in mind that this still does not address 
the problem that one is still using this to estimate what is 
reproducible in a reconstruction and what is noise. This becomes most 
obvious if this measure is applied to a structure where symmetry has 
been imposed, but I believe that it is perfectly general. If one imposes 
the wrong symmetry, one can still measure a self-consistency between two 
different reconstructions, but this has no reality in terms of actual 
resolution (Egelman, eLife, 2015).
Regards,
Ed

On 8/12/15 8:51 AM, Frank, Joachim wrote:
> IT DOESN'T.
>
> Joachim.
>

-- 


Edward H. Egelman, Ph.D.

Professor

Dept. of Biochemistry and Molecular Genetics

University of Virginia

President

Biophysical Society

phone: 434-924-8210

fax: 434-924-5069

egelman at virginia.edu

http://www.people.virginia.edu/~ehe2n 
<http://www.people.virginia.edu/%7Eehe2n>


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