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Since Marin brought up the can of worms metaphor, let me just add my
own two bits: FSC has never been a measure of resolution (in the
Rayleigh sense of an instrument's ability two resolve two points)
but has been a measure of self-consistency. One can then argue about
the statistics of this measure, but please keep in mind that this
still does not address the problem that one is still using this to
estimate what is reproducible in a reconstruction and what is noise.
This becomes most obvious if this measure is applied to a structure
where symmetry has been imposed, but I believe that it is perfectly
general. If one imposes the wrong symmetry, one can still measure a
self-consistency between two different reconstructions, but this has
no reality in terms of actual resolution (Egelman, eLife, 2015).<br>
Regards,<br>
Ed<br>
<br>
<div class="moz-cite-prefix">On 8/12/15 8:51 AM, Frank, Joachim
wrote:<br>
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<div>Joachim.</div>
<br>
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<p class="MsoNormal">Edward H. Egelman, Ph.D.<o:p></o:p></p>
<p class="MsoNormal">Professor<o:p></o:p></p>
<p class="MsoNormal">Dept. of Biochemistry and Molecular
Genetics<o:p></o:p></p>
<p class="MsoNormal">University of Virginia<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">President<o:p></o:p></p>
<p class="MsoNormal">Biophysical Society<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">phone: 434-924-8210<o:p></o:p></p>
<p class="MsoNormal">fax: 434-924-5069<o:p></o:p></p>
<p class="MsoNormal"><a class="moz-txt-link-abbreviated" href="mailto:egelman@virginia.edu">egelman@virginia.edu</a><o:p></o:p></p>
<p class="MsoNormal"><a
href="http://www.people.virginia.edu/%7Eehe2n"><a class="moz-txt-link-freetext" href="http://www.people.virginia.edu/~ehe2n">http://www.people.virginia.edu/~ehe2n</a></a><o:p></o:p></p>
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