[3dem] getting protein samples into holes in quantifoil

Gabriel Lander glander at gmail.com
Thu Oct 30 23:24:23 PDT 2014


This exact question was just discussed in March of this year, and probably a dozen times before that in the past. It’s a very common problem with no simple solution.
https://mail.ncmir.ucsd.edu/pipermail/3dem/2014-March/003276.html <https://mail.ncmir.ucsd.edu/pipermail/3dem/2014-March/003276.html>

> On Oct 30, 2014, at 10:58 PM, Sheemei Lok <sheemei.lok at duke-nus.edu.sg> wrote:
> 
> Dear all,
> Our protein sample tend to stick to the carbon film instead of the hole in quantifoil. Are there any tricks that we can do to get them into the holes?
> 
> Regards,
> Shee-Mei Lok
> Associate Professor
> Program in Emerging Infectious Diseases,
> Duke-NUS Graduate Medical School,
> KTP Building
> Level 9 Room 19
> 8 college Road,
> Singapore 169857
> Ph: 65-65165840
> Fax:65-62212529
> email: sheemei.lok at duke-nus.edu.sg <applewebdata://E5F18255-3202-4C1B-877E-34E4A73B3779/sheemei.lok@duke-nus.edu.sg> 
> 
> 
> 
> 
> 
> 
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