[3dem] Loosing tilt angle information by dm4Tomrc conversion

[Christian Geiss]

Dear 3dem group,

I found a solution as a colleague was directing me to...after this, it 
is kind of trivial, at least for experienced IMOD users, for those like 
me, who don't use it regularily, here comes the easy solution:

In the initial setup of etomo, you can manually set the angular range 
and increment by specifiying the starting angle option. Somehow, I have 
overseen this.

@ Steve

Ok, it must be that I then run a older version.

@ Reinhard

Thanks for this information. Next time, I will adress more IMOD-specific 
questions to this email list.

Thanks for your contribution,
Christian


On 11/14/2014 03:34 PM, Steven Ludtke wrote:
> Hi Christian, the current version of EMAN2.1 should be able to read 
> DM4 images/stacks and headers. What version were you trying ?  If you 
> are having a problem with this please let us know as it should be 
> working properly.
>
> ----------------------------------------------------------------------------
> Steven Ludtke, Ph.D.
> Professor, Dept. of Biochemistry and Mol. Biol.                Those 
> who do
> Co-Director National Center For Macromolecular Imaging           ARE
> Baylor College of Medicine The converse
> sludtke at bcm.edu <mailto:sludtke at bcm.edu>  -or- 
> stevel at alumni.caltech.edu <mailto:stevel at alumni.caltech.edu>           
>     also applies
> http://ncmi.bcm.edu/~stevel <http://ncmi.bcm.edu/%7Estevel>
>
>
>
>> On Nov 14, 2014, at 3:49 AM, Christian Geiss <geiss at biophysik.org 
>> <mailto:geiss at biophysik.org>> wrote:
>>
>> Dear 3dem-Group,
>>
>> I am looking for a decent way to manipulate the header information in 
>> MRC files, particularly the metadata where e.g. tilt angles are 
>> saved, since
>> IMOD cannot generate the com scripts in the initial setup when 
>> running the extracttilts subroutine. Has anyone experience with this?
>>
>> The background is that I loose my tilt angle information when 
>> converting dm4 files to mrc by the dm2mrc routine of the imod 
>> package. It would be also quite useful if someone knows a conversion 
>> script that preserves this information (EM2EM, EMAN2 for instance are 
>> all not versatile to this as they do not support dm4 or they cannot 
>> handle dm4 dose fractionation stacks).
>>
>> Thanks in advance,
>> Christian Geiss
>> -- 
>> *Christian Geiss, M.sc.*
>> Universitaet Frankfurt BMLS
>> Frangakis Group
>> Max-von-Laue-Strasse 15
>> D - 60438 Frankfurt am Main
>> Phone: +49-(0)69-79842597
>> Fax: +49-(0)69-79846467
>> geiss at biophysik.org
>> _______________________________________________
>> 3dem mailing list
>> 3dem at ncmir.ucsd.edu <mailto:3dem at ncmir.ucsd.edu>
>> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
>


-- 
*Christian Geiss, M.sc.*
Universitaet Frankfurt BMLS
Frangakis Group
Max-von-Laue-Strasse 15
D - 60438 Frankfurt am Main
Phone: +49-(0)69-79842597
Fax: +49-(0)69-79846467
geiss at biophysik.org
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