[3dem] Merging datasets with different pixel sizes

Philip Köck Philip.Koeck at ki.se
Wed Nov 12 22:45:00 PST 2014


That's a difference of less than 2 percent.
Do you really know the magnification so exactly?
And would 2 percent make any difference to the final resolution?

Philip


-----Ursprungligt meddelande-----
Från: 3dem-bounces at ncmir.ucsd.edu [mailto:3dem-bounces at ncmir.ucsd.edu] För Carlos Oscar S. Sorzano
Skickat: den 13 november 2014 07:08
Till: Meyerson, Joel (NIH/NCI) [F]; 3dem at ncmir.ucsd.edu
Ämne: Re: [3dem] Merging datasets with different pixel sizes

Dear Joel,

you may use xmipp_transform_geometry with --scale 1.0188 (=0.650/0.638). 
It uses cubic B-spline for the interpolation, which is rather accurate.

Kind regards, Carlos Oscar

On 11/13/2014 12:20 AM, Meyerson, Joel (NIH/NCI) [F] wrote:
> Dear All,
> I have two K2 datasets, one with an unbinned apix of 0.638 and another with unbinned apix of 0.650. Can someone suggest an approach to re-scaling one or both of the datasets such that they can be merged for processing? In case it's relevant, I intend to process the datasets in Relion.
>
> Thank you,
> Joel
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> 3dem at ncmir.ucsd.edu
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>

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Carlos Oscar Sánchez Sorzano                  e-mail:   coss at cnb.csic.es
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