[3dem] Sunday short course at M&M 2014 Aug 3-7, Hartford,CT

Michael Radermacher Michael.Radermacher at uvm.edu
Mon May 19 06:49:48 PDT 2014

Dear All,

As the deadline for early registration for this year’s Microscopy and  
Microanalysis Meeting (Aug. 3-7 2014 in Hartford, CT) is coming  
closer, I would like to make you aware of our Sunday Short Course on  
3D electron microscopy (description below). Please forward this also  
to your colleagues who may be interested in this topic.
This course is ideal for people who need a comprehensive introduction  
or a refresher in 3D reconstruction methods and is suitable for  
biologists and material scientists.
If you have already registered for the meeting you can add the course  
registration as  “extra item only”.

X12 3D Electron Microscopy of Macromolecular Assemblies
Teresa Ruiz, Michael Radermacher, Edward Morris
8:30 AM to 5:00 PM on Sunday, August 3. 2014

•	Sample preparation: deep stain, vitreous ice
•	Imaging conditions, low-dose imaging, tilt-pair data collection
•	Alignment techniques and multivariate statistical analysis
•	3D reconstruction methods
•	X-ray structure docking
•	Techniques described have applications in both biological and  
material science

This short course will provide a comprehensive description of the  
methods used for 3D structure determination from electron micrographs  
of macromolecular complexes or weakly scattering specimens available  
in multiple copies. Specimen-preparation techniques for single  
particles (deep stain, vitreous ice) will be presented, followed by  
selection of optimal imaging conditions, including low-dose imaging.  
Next, a detailed explanation of image-processing techniques, with  
special emphasis on the random-conical reconstruction technique, will  
be presented. Finally, structure interpretation and docking of X-ray  
structures to 3D EM densities will be demonstrated. The techniques  
described could be applied to both biological and materials science  

Michael Radermacher, Ph.D., Prof., FMSA
University of Vermont
Dept. Molecular Physiology and Biophysics
HSRF 120 / 149 Beaumont Ave
Burlington, VT 05405

Tel: + 802 656-4834
Fax: + 802 656-0747

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