[3dem] Unrecognised CryoEM contaminant 2

[Hampton, Cheri]

Hi, I wonder could it be something in your buffer? It surrounds the spheres rather than laying on top. If the sample is dry from evaporation rather than blotting, strange things can precipitate.

Cheri Hampton, PhD
Emory University
914-413-8809


On Mar 28, 2014, at 8:53 AM, "Daniel Luque" <dluque at cnb.csic.es<mailto:dluque at cnb.csic.es>> wrote:

Hello

Before a couple of days waiting for the list moderator approval to attach the images in the list , I do not have received any response (either positive or negative) and I do not have find a mail/way for contact. Thus, I have made the images available at our server at:

http://halley.cnb.csic.es/~virus/3DEM/Example_images.pdf

Bellow is text of the original question:

We are doing CryoEM of viral particles. When we check the sample by negative staining, a field of intact homogeneous particles without significant contaminants is observed. However, when we visualize the same sample by CryoEM, additionally to a lower-than expected number of particles, we observe an unexpected contaminant mostly located in the holes (see attached document with some example images). Does anybody see this class of contaminants? Any idea about its origin?

Thanks in advance!


Daniel Luque




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