[3dem] Unrecognised CryoEM contaminant 2

[Xinghong Dai]

Hi Daniel,

To me, it looks like phantom caused by too thin ice. Especially black
patches around the hole in up-left panel, they look like almost-dry areas.
Try to increase the humidity, and wait shorter time before, and after, you
blot. Also try shorter blot time. 

Good luck!

 

Best regards, 

 

 

Xinghong Dai



发件人: 3dem-bounces at ncmir.ucsd.edu [mailto:3dem-bounces at ncmir.ucsd.edu] 代
表 Daniel Luque
发送时间: Friday, March 28, 2014 5:52 AM
收件人: 3dem at ncmir.ucsd.edu
主题: [3dem] Unrecognised CryoEM contaminant 2

 

Hello

 

Before a couple of days waiting for the list moderator approval to attach
the images in the list , I do not have received any response (either
positive or negative) and I do not have find a mail/way for contact. Thus, I
have made the images available at our server at:

 

http://halley.cnb.csic.es/~virus/3DEM/Example_images.pdf

 

Bellow is text of the original question:

 

We are doing CryoEM of viral particles. When we check the sample by negative
staining, a field of intact homogeneous particles without significant
contaminants is observed. However, when we visualize the same sample by
CryoEM, additionally to a lower-than expected number of particles, we
observe an unexpected contaminant mostly located in the holes (see attached
document with some example images). Does anybody see this class of
contaminants? Any idea about its origin?

 

Thanks in advance!

 

 

Daniel Luque

 

 

 

 

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