[3dem] Unrecognised CryoEM contaminant 2

[Vladan Lucic]

Hi Daniel,

The contamination you see might be related to what was discussed on this list 
some time ago. Perhaps if would help to find out if the amount of 
contamination you see increases with the time spent in column. In our case it 
didn't increase and we could get rid of it by making ethane bit warmer.

Good luck,

Vladan

-- 
Vladan Lucic, PhD
Max Planck Institute of Biochemistry
Department of Molecular Structural Biology
Am Klopferspitz 18
82152 Martinsried
Germany

Phone +49 89 8578-2647
Web: http://www.biochem.mpg.de/277447/15_ContentSynCompl


On Friday 28 March 2014 13:52:27 Daniel Luque wrote:
> Hello
> 
> 
> 
> Before a couple of days waiting for the list moderator approval to attach
> the images in the list , I do not have received any response (either
> positive or negative) and I do not have find a mail/way for contact. Thus, I
> have made the images available at our server at:
> 
> 
> 
> http://halley.cnb.csic.es/~virus/3DEM/Example_images.pdf
> 
> 
> 
> Bellow is text of the original question:
> 
> 
> 
> We are doing CryoEM of viral particles. When we check the sample by negative
> staining, a field of intact homogeneous particles without significant
> contaminants is observed. However, when we visualize the same sample by
> CryoEM, additionally to a lower-than expected number of particles, we
> observe an unexpected contaminant mostly located in the holes (see attached
> document with some example images). Does anybody see this class of
> contaminants? Any idea about its origin?
> 
> 
> 
> Thanks in advance!
> 
> 
> 
> 
> 
> Daniel Luque