[3dem] Northern California Society for Microscopy 2014 Spring Meeting

Roseann Csencsits rcsencsits at lbl.gov
Fri Mar 28 07:54:53 PDT 2014


The Northern California Society for Microscopy Spring Meeting, featuring presentations by Kent McDonald and Ryan McGorty, will be held Thursday, April 17, 2014 6:00 PM to 9:00 PM.

Please RSVP to info at ncsmicroscopy.org

Scotts of Palo Alto
#1 Town and Country Village
Palo Alto, California
http://scottsseafoodpa.com/


Featured Speakers Are: 


Kent McDonald, PhD, Electron Microscopy Lab, UC Berkeley

Rapid Specimen Processing Methods for Cryofixed Samples

New methods for freeze substitution have been developed that
require only basic laboratory tools. Excellent freeze substitution
results can be obtained in as little as 90min for cells of small
volume. Rapid epoxy resin infiltration can be achieved by centrifugation
followed by polymerization at 100 C for 1.5–2 h. Total processing time
from freezing to blocks ready to section is about 6 h. Comparative
results will be presented.


Ryan McGorty, PhD, UC San Francisco, School of Pharmacy

Three-dimensional super-resolution imaging of tissue samples

Single-molecule localization microscopy methods are often used to
study samples at or very near the coverslip surface with a spatial
resolution of around 20nm. However, a number of challenges emerge when
trying to image even a few microns past the coverslip surface,
particularly when trying to accurately localize molecules in 3D. After
first explaining the principle of the super-resolution microscopy
technique we use, I will discuss multiple methods we have recently
developed that allow us to image deeper into tissue samples.


No Cost to Current Members. $25 for Non-Members.

Bring your check, payable to NCSM, for 2014 dues and enjoy a 3-course meal and great speakers.
Annual dues for regular members are $40; students, $20; and a
corporate membership (which includes one person) is $100, payable to "NCSM"



_______________________
Roseann Csencsits, PhD
Scientist in Charge, Downing TEM Tools
Lawrence Berkeley Lab 01-365
1 Cyclotron Road
Berkeley, CA 94720
510-486-4548














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