[3dem] Unrecognised CryoEM contaminant

[DANIEL.LUQUE.BUZO.40704]

  Hello,

Sorry, I forgot to attach the document. I have resent it to the list but is
pending for the list moderator aprobal because the document is bigger that
40 Kb.

Thanks

Daniel

Daniel Luque <dluque at cnb.csic.es> escribió:

> Hello,
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>      
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>     We are doing CryoEM of viral particles. When we check the sample by
> negative staining, a field of intact homogeneous particles without
> significant contaminants is observed. However, when we visualize the
> same sample by CryoEM, additionally to a lower-than-expected number of
> particles, we observe an unexpected contaminant mostly located in the
> holes (see attached document with some example images). Does anybody see
> this class of contaminants? Any idea about its origin?
>
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>
>     Thanks in advance!
>
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>     Daniel Luque
>
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>      
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>      
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