[3dem] Virus on Carbon

[Luiza Mendonça]

Dear colleagues,

I work with HIV particles and (at least in my samples), they like the
carbon film on the grids much better than the holes, but for me this is a
big problem, as, even using high titer preps, they rarely go to the holes,
resulting in less-than-perfect tomograms. Has anyone else had this problem?
What did you do to solve it?
My particles are resusspended in PBS (with gold fiducials) at the time of
freezing.

Best,
Luiza.
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