[3dem] Tenure Track Position available in Correlative Light and Electron Microscopy Imaging in the Koster Lab

[A.J.Koster at lumc.nl]

Tenure Track Position available in Correlative Light and Electron Microscopy Imaging

A tenure track position is available immediately in laboratory of Bram Koster at the Leiden University Medical Centre (LUMC) in Leiden, the Netherlands (https://electronmicroscopy.lumc.nl/). 

The Koster lab uses both light and electron microscopy methods to investigate various cell biological questions. Correlative light and electron microscopy (CLEM) is a technical focus area within the laboratory, together with the application and development of both cryo EM and traditional resin-embedding techniques to examine macromolecular structures, cells and tissue. The Koster laboratory is closely linked the Netherlands Center for Electron Nanoscopy (NeCEN, https://www.necen.nl). Excellent facilities are available at NeCEN to investigate questions related to structural biology.

Our lab is ideally equipped for high-throughput and high-resolution EM and CLEM. We have three TEMs available at the LUMC with various attachments; an F20 with 4k Gatan CCD, 2002 GIF with 4k CCD and an FEI iCorr light microscopy unit, and two T12s with 4k Eagle CCDs, one of which is equipped with STEM and an iLEM light microscope unit. We also have an SEM and in-house equipment for specimen preparation, including: Leica EMPACT2, Leica cryo plunger, MAVIS light microscopy plunger, two automated freeze substitution units, and two cryo-ultramicrotomes. All our microscopes are equipped with automated 2D and 3D imaging software. We have a Linkam CMS 196 light microscopy stage suitable for imaging EM specimen at cryogenic conditions. We also have access to the LUMC advanced light microscopy core facility that is housed within our department, including super-resolution systems (STED).

At NeCEN there are two Titan Krios TEMs with direct electron detectors available for our use. One Titan is equipped with a Falcon 2 detector and is being upgraded with an additional GIF equipped with a K2 detector and a phase plate. The other Titan has a Cs corrector and a Falcon 2 detector. NeCEN is aimed at providing data collection equipment for high resolution imaging for both single particle studies and tomography. NeCEN is also closely affiliated with other institutes and universities, which provides excellent opportunities for collaborations.

The successful candidate should have a PhD and a proven post-doctoral track record in structural biology, cell biology or biophysics and have previous experience with light and/or electron (cryo)-microscopy. It will be advantageous if the candidate has proven interest in method development. 

The ideal candidate would have a research proposal that fits at least one of the seven research profile areas of the LUMC (https://www.lumc.nl/home/0002/80123045005221/) and for which extensive use of the advanced microscopy infrastructure at the LUMC and NeCEN is expected to contribute.

Candidates should contact Dr. Bram Koster (a.j.koster at lumc.nl) and include a CV, statement of research interests, and contact information for three references.