[3dem] electron dose & data collection strategy for Titan Krios, 300kV, Falcon back thinned

[Shabih Shakeel]

Dear All,

I am going to collect single particle data on Titan Krios operated at 300kV
with Falcon II (back-thinned) detector. The sample is icosahedral virus of
30nm diameter. Does any one have experience collecting data in
these settings?

We are especially looking for comments or advice on electron dosage to use
and number of frames to record with an aimed resolution of 3-5Å. This is
our one shot at collecting the data for this sample, so we really want it
to work.

Of course, any other useful comments, advice, tips would also be
appreciated.

Thanks a lot.

Best Regards,
Shabih Shakeel
-- 
Shabih Shakeel, MSc
Doctoral researcher
Sarah Butcher group
Institute of Biotechnology
P.O. Box 65 (Viikinkaari 1)
00014 University of Helsinki
Finland

cell: +358 46 8430774
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