[3dem] Vitrobot Mark IV water cylinder

[Bob Grassucci]

Hi All,
I just wanted to follow up now that I have a second.  The problem turned 
out to be a faulty fan that distributes the water vapor in the chamber.  
We got a new one from a computer supply site for about $20 and after a 
little diss assembly and reassembly we are back in business.  Thank you 
for all your helpful suggestions.  I learned a lot and am glad it was a 
inexpensive repair.
Regards,
Bob

On 12/18/2013 5:21 PM, Bob Grassucci wrote:
> Hi All,
> Thanks for all of your helpful suggestions.  Below is what I have 
> tried and my conclusions.
> 1 There is no leak in the water cylinder that would cause it to empty.
> 2 The sonicator is working (I took off the cylinder and sprayed myself 
> with water.
> 3 I can hear the fan so it should be dispersing the water vapor.
> 4 I can check the humidity sensor but doubt that is the problem since 
> it gets there at times.
> 5 I set the humidifier to manual and got little puffs of humidity 
> rather than blowing like crazy.
> 6. This is at 6 degrees so it does take a while to bring it to 100% 
> since the humidity condenses on the cold surfaces.
> 7 When I mount the cylinder and pull with the syringe I get very 
> little resistance making me suspect a poor seal with the chamber thus 
> not pulling water from the outer to inner vessel.
>
> Thanks again for your help.
> Bob
>
>
> On 12/18/2013 11:47 AM, Bob Grassucci wrote:
>> Hi All,
>> We are having a problem getting the humidity up to 100% on our 
>> Vitrobot Mark IV and expect it might have something to do with the 
>> water cylinder or it's seal to where it is mounted.  The water 
>> container seems a bit looser than what I remember and suspect this 
>> may have something to do with the humidity problem.  My question to 
>> the list is has anyone else had this problem and if so how did you 
>> solve it?  Thanks.
>> Bob
>>
>>
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