[3dem] Vitrobot Mark IV water cylinder
rg2502 at columbia.edu
Tue Feb 11 18:23:34 PST 2014
I just wanted to follow up now that I have a second. The problem turned
out to be a faulty fan that distributes the water vapor in the chamber.
We got a new one from a computer supply site for about $20 and after a
little diss assembly and reassembly we are back in business. Thank you
for all your helpful suggestions. I learned a lot and am glad it was a
On 12/18/2013 5:21 PM, Bob Grassucci wrote:
> Hi All,
> Thanks for all of your helpful suggestions. Below is what I have
> tried and my conclusions.
> 1 There is no leak in the water cylinder that would cause it to empty.
> 2 The sonicator is working (I took off the cylinder and sprayed myself
> with water.
> 3 I can hear the fan so it should be dispersing the water vapor.
> 4 I can check the humidity sensor but doubt that is the problem since
> it gets there at times.
> 5 I set the humidifier to manual and got little puffs of humidity
> rather than blowing like crazy.
> 6. This is at 6 degrees so it does take a while to bring it to 100%
> since the humidity condenses on the cold surfaces.
> 7 When I mount the cylinder and pull with the syringe I get very
> little resistance making me suspect a poor seal with the chamber thus
> not pulling water from the outer to inner vessel.
> Thanks again for your help.
> On 12/18/2013 11:47 AM, Bob Grassucci wrote:
>> Hi All,
>> We are having a problem getting the humidity up to 100% on our
>> Vitrobot Mark IV and expect it might have something to do with the
>> water cylinder or it's seal to where it is mounted. The water
>> container seems a bit looser than what I remember and suspect this
>> may have something to do with the humidity problem. My question to
>> the list is has anyone else had this problem and if so how did you
>> solve it? Thanks.
>> 3dem mailing list
>> 3dem at ncmir.ucsd.edu
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> 3dem at ncmir.ucsd.edu
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